A new non-radioactive assay of phytoene desaturase to evaluate bleaching herbicides
- A non-radioactive cell-free assay was developed to quantitatively determine inhibition of plant-type phytoene desaturase by bleaching herbicides. An active desaturase was prepared from an appropriately cloned E. coli transformant. Another E. coli transformant was used to produce the required phytoene. Phytofluene and t-carotene, the products of the desaturase reaction, were either determined by HPLC or optical absorption spectra. Enzyme kinetics and inhibition data for the bleaching tetrazole herbicide WL110547 are presented as an example.
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| Author: | Gerhard SandmannORCiD, Christian Schneider, Peter BögerGND |
|---|---|
| URN: | urn:nbn:de:hebis:30:3-799425 |
| DOI: | https://doi.org/10.1515/znc-1996-7-812 |
| ISSN: | 0939-5075 |
| ISSN: | 1865-7125 |
| Parent Title (German): | Zeitschrift für Naturforschung, C |
| Publisher: | Verlag der Zeitschrift für Naturforschung |
| Place of publication: | Tübingen |
| Document Type: | Article |
| Language: | English |
| Date of Publication (online): | 2014/06/02 |
| Year of first Publication: | 1996 |
| Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
| Release Date: | 2024/02/06 |
| Tag: | I50 Value; Phvtoene Desaturase; Phvtofluene; Phytoene; WL110547; in-vitro Assay; ζ-Carotene |
| Volume: | 51.1996 |
| Issue: | 7-8 |
| Page Number: | 5 |
| First Page: | 534 |
| Last Page: | 538 |
| Institutes: | Biowissenschaften |
| Biochemie, Chemie und Pharmazie / Biochemie und Chemie | |
| Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
| Sammlungen: | Universitätspublikationen |
| Licence (German): |  Creative Commons - Namensnennung-Nicht kommerziell-Keine Bearbeitung 3.0 |
