Conserved properties of polypeptide transport-associated (POTRA) domains derived from cyanobacterial Omp85

  • Proteins of the Omp85 family are conserved in all kingdoms of life. They mediate protein transport across or protein insertion into membranes and reside in the outer membranes of Gram-negative bacteria, mitochondria, and chloroplasts. Omp85 proteins contain a C-terminal transmembrane β-barrel and a soluble N terminus with a varying number of polypeptide-transport-associated or POTRA domains. Here we investigate Omp85 from the cyanobacterium Anabaena sp. PCC 7120. The crystallographic three-dimensional structure of the N-terminal region shows three POTRA domains, here named P1 to P3 from the N terminus. Molecular dynamics simulations revealed a hinge between P1 and P2 but in contrast show that P2 and P3 are fixed in orientation. The P2-P3 arrangement is identical as seen for the POTRA domains from proteobacterial FhaC, suggesting this orientation is a conserved feature. Furthermore, we define interfaces for protein-protein interaction in P1 and P2. P3 possesses an extended loop unique to cyanobacteria and plantae, which influences pore properties as shown by deletion. It now becomes clear how variations in structure of individual POTRA domains, as well as the different number of POTRA domains with both rigid and flexible connections make the N termini of Omp85 proteins versatile adaptors for a plentitude of functions.

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Author:Patrick KönigGND, Oliver MirusGND, Raimund Haarmann, Maik Sascha SommerGND, Irmgard SinningORCiDGND, Enrico SchleiffORCiDGND, Ivo TewsORCiDGND
URN:urn:nbn:de:hebis:30:3-765001
DOI:https://doi.org/10.1074/jbc.M110.112649
ISSN:0021-9258
Pubmed Id:https://pubmed.ncbi.nlm.nih.gov/20348103
Parent Title (English):Journal of biological chemistry
Publisher:American Society for Biochemistry and Molecular Biology Publications
Place of publication:Bethesda, Md
Document Type:Article
Language:English
Date of Publication (online):2021/01/04
Year of first Publication:2010
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2024/04/17
Tag:Bacteria; Evolution; Membrane Biogenesis; Membrane Proteins; POTRA Domains; Protein Structure; Protein Translocation
Volume:285.2010
Issue:23
Page Number:9
First Page:18016
Last Page:18024
Institutes:Biowissenschaften
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
Sammlungen:Universitätspublikationen
Licence (German):License LogoCreative Commons - CC BY - Namensnennung 4.0 International