An improved assay of UV-induced thymine-containing dimers in Saccharomyces cerevisiae

  • The method for assaying thymine-containing dimers in yeast is based on highly efficient ([3H]-deoxythymidine-5′-monophosphate) DNA-specific labelling and employs ascending thin layer chromatography. It allows satisfactory quantitative analysis down to UV-doses of 500 erg/mm2.

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Metadaten
Author:Wolfgang Walter Fäth, Martin BrendelORCiD
URN:urn:nbn:de:hebis:30:3-722374
DOI:https://doi.org/10.1515/znc-1975-11-1220
ISSN:0939-5075
ISSN:1865-7125
Parent Title (German):Zeitschrift für Naturforschung, C
Publisher:Verlag der Zeitschrift für Naturforschung
Place of publication:Tübingen
Document Type:Article
Language:English
Date of Publication (online):2014/06/02
Year of first Publication:1975
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2024/02/02
Tag:DNA-Specific Labelling; Thin Layer Chromatography; Thymine Dimers
Volume:30.1975
Issue:11-12
Page Number:7
First Page:804
Last Page:810
Institutes:Biowissenschaften
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
Sammlungen:Universitätspublikationen
Licence (German):License LogoCreative Commons - Namensnennung-Nicht kommerziell-Keine Bearbeitung 3.0