Activation of chromatin-bound DNA-dependent RNA polymerase (E.C. 2.7.7.6.) in plant storage tissue slices

  • Chromatin, RNA Polymerase, Potato Tuber Tissue, Aging Phenomenon The synthesis of RNA by chromatin-bound RNA polymerase (E.C. 2.7.7.6.) from white potato tubers proceeds at a low rate, which is enhanced after slicing the tissue, however. Concomitantly DNA template availability as measured with saturating amounts of Escherichia coli polymerase is diminished drastically. Nearest neighbor frequency analysis proved that the RNA synthesized on chromatin of intact tubers is different from that synthesized on chromatin of sliced tissue. The RNA polymerase of white potato tubers is dependent on all four ribonucleoside triphos­ phates and a divalent metal ion such as Mg2+ or Mn2+ and totally inhibited by the presence of pyrophosphate. Actinomycin D blocks the formation of the RNA product, which could be shown to be a heteropolymer by nearest neighbour frequency technique. The Km of the chromatin-bound enzyme with regard to ATP, GTP, CTP and UTP was 5.1 X10-5 M, 1.6X10-5 M, 0.9X10-5 M and 0.45 X 10-5M/1 respectively, α-amanitin inhibits the overall activity to about 50%, which indicates the presence of equal amounts of polymerase I and polymerase If.

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Metadaten
Author:Günter KahlGND, Manfred Wechselberger
URN:urn:nbn:de:hebis:30:3-720330
DOI:https://doi.org/10.1515/znc-1977-3-413
ISSN:0939-5075
ISSN:1865-7125
Parent Title (German):Zeitschrift für Naturforschung, C
Publisher:Verlag der Zeitschrift für Naturforschung
Place of publication:Tübingen
Document Type:Article
Language:English
Date of Publication (online):2014/06/02
Year of first Publication:1977
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2024/01/31
Tag:Aging Phenomenon; Chromatin; Potato Tuber Tissue; RNA Polymerase
Volume:32.1977
Issue:3-4
Page Number:7
First Page:229
Last Page:235
Institutes:Biowissenschaften
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
Sammlungen:Universitätspublikationen
Licence (German):License LogoCreative Commons - Namensnennung-Nicht kommerziell-Keine Bearbeitung 3.0