Fewer functional deficits and reduced cell death after ranibizumab treatment in a retinal ischemia model
- Retinal ischemia is an important factor in several eye disorders. To investigate the impact of VEGF inhibitors, as a therapeutic option, we studied these in a retinal ischemia animal model. Therefore, animals received bevacizumab or ranibizumab intravitreally one day after ischemia induction. Via electroretinography, a significant decrease in a- and b-wave amplitudes was detected fourteen days after ischemia, but they were reduced to a lesser extent in the ranibizumab group. Ischemic and bevacizumab retinae displayed fewer retinal ganglion cells (RGCs), while no significant cell loss was noted in the ranibizumab group. Apoptosis was reduced after therapy. More autophagocytotic cells were observed in ischemic and bevacizumab eyes, but not in ranibizumab eyes. Additionally, more microglia, as well as active ones, were revealed in all ischemic groups, but the increase was less prominent under ranibizumab treatment. Fewer cone bipolar cells were detected in ischemic eyes, in contrast to bevacizumab and ranibizumab-treated ones. Our results demonstrate a reduced apoptosis and autophagocytosis rate after ranibizumab treatment. Furthermore, a certain protection was seen regarding functionality, RGC, and bipolar cell availability, as well as microglia activation by ranibizumab treatment after ischemic damage. Thus, ranibizumab could be an option for treatment of retinal ischemic injury.
Author: | Marina PalmhofGND, Stephanie LohmannGND, Dustin SchulteGND, Gesa StuteGND, Natalie WagnerGND, Burkhard DickORCiDGND, Stephanie Christine JoachimORCiDGND |
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URN: | urn:nbn:de:hbz:294-78240 |
DOI: | https://doi.org/10.3390/ijms19061636 |
Parent Title (English): | International journal of molecular sciences |
Publisher: | MDPI |
Place of publication: | Basel |
Document Type: | Article |
Language: | English |
Date of Publication (online): | 2021/02/03 |
Date of first Publication: | 2018/05/31 |
Publishing Institution: | Ruhr-Universität Bochum, Universitätsbibliothek |
Tag: | Open Access Fonds ERG; VEGF; apoptosis; autophagocytosis; bevacizumab; ischemia/reperfusion; ranibizumab; retina; retinal ganglion cell |
Volume: | 19 |
Issue: | 6, Article 1636 |
First Page: | 1636-1 |
Last Page: | 1636-19 |
Note: | Article Processing Charge funded by the Deutsche Forschungsgemeinschaft (DFG) and the Open Access Publication Fund of Ruhr-Universität Bochum. |
Institutes/Facilities: | Experimental Eye Research Institute |
Knappschaftskrankenhaus Bochum, Klinik für Augenheilkunde | |
Dewey Decimal Classification: | Technik, Medizin, angewandte Wissenschaften / Medizin, Gesundheit |
open_access (DINI-Set): | open_access |
Licence (English): | Creative Commons - CC BY 4.0 - Attribution 4.0 International |