Structural and functional characterization of prokaryotic CPX-ATPases
Strukturelle und funktionelle Charakterisierung von prokaryiotischen CPx-ATPasen
- P-type ATPases are integral membrane proteins that facilitate the active transport of cations across membranes driven by ATP-hydrolysis. One subfamily is formed by CPx-ATPases that fulfill a crucial role in heavy metal homoeostasis. Several prokaryotic CPx-ATPase genes have been expressed in the host strain \(\textit {Escherichia coli}\) BL21 C41. Homogenous preparations of the recombinant proteins can be obtained by the devised purification scheme. The metal substrate specifity can be predicted for most of the recombinant CPx-ATPases, which is not possible for the \(\textit {Sulfolobus solfataricus}\) CopB protein. Purified CopB exhibits a vanadate-sensitive,Co\(^{2+}\)-dependent ATPase activity. CopB ATPase activity is optimal at 70° and is strictly dependent on the presence of lipids. Inside-out membrane vesicles of \(\textit {E. coli}\) expressing CopB accumulate \(^{57}\)Co upon addition of ATP, indicating a genuine export function. The cobalt exporter CopB of \(\textit {S. solfataricus}\) may be representative of a novel subgroup of P-type ATPases.
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| Author: | Martin Zoltner |
|---|---|
| URN: | urn:nbn:de:hbz:294-22162 |
| Subtitle (English): | evidence for a novel cobalt exporting P-type ATPase |
| Subtitle (German): | Beweis für eine neuartige Kobalt exportierende P-Type ATPase |
| Referee: | Mathias LübbenGND, Arnold J. M. DriessenGND |
| Document Type: | Doctoral Thesis |
| Language: | English |
| Date of Publication (online): | 2008/05/14 |
| Date of first Publication: | 2008/05/14 |
| Publishing Institution: | Ruhr-Universität Bochum, Universitätsbibliothek |
| Granting Institution: | Ruhr-Universität Bochum, Fakultät für Biologie und Biotechnologie |
| Date of final exam: | 2007/02/02 |
| Creating Corporation: | Ruhr-Universität Bochum, Fakultät für Biologie und Biotechnologie |
| GND-Keyword: | P-Typ-ATPasen; Schwermetall; Schwermetall / Transport; Membranproteine |
| Institutes/Facilities: | Lehrstuhl für Biophysik |
| Dewey Decimal Classification: | Naturwissenschaften und Mathematik / Biowissenschaften, Biologie, Biochemie |
| Licence (German): |  Creative Commons - CC BY-NC-ND 3.0 - Namensnennung-Nicht kommerziell-Keine Bearbeitung |
