- AutorIn
- Jana Breitfeld
- Stephanie Kehr
- Luise Müller
- Peter F. Stadler
- Yvonne Böttcher
- Matthias Blüher
- Michael Stumvoll
- Peter Kovacs
- Titel
- Developmentally Driven Changes in Adipogenesis in Different Fat Depots Are Related to Obesity
- Zitierfähige Url:
- https://nbn-resolving.org/urn:nbn:de:bsz:15-qucosa2-845537
- Quellenangabe
- Frontiers in endocrinology
Erscheinungsjahr: 2020
Jahrgang: 11
E-ISSN: 1664-2392
Artikelnummer: 138 - Erstveröffentlichung
- 2020
- Abstract (EN)
- Subcutaneous (sc) and visceral (vis) adipose tissue (AT) contribute to the variability in pathophysiological consequences of obesity and adverse fat distribution. To gain insights into the molecular mechanisms distinguishing vis and sc fat, we compared the transcriptome during differentiation of immortalized adipocytes from murine epididymal (epi) and inguinal (ing) AT. RNA was extracted on different days of adipogenesis (−2, 0, 2, 4, 6, 8) and analyzed using ClariomTM D mouse assays (Affymetrix) covering >214,900 transcripts in >66,100 genes. Transcript Time Course Analysis revealed 137 differentially expressed genes. The top genes with most divergent expression dynamics included developmental genes like Alx1, Lhx8, Irx1/2, Hoxc10, Hoxa5/10, and Tbx5/15. According to pathway analysis the majority of the genes were enriched in pathways related to AT development. Finally, in paired samples of human vis and sc AT (N = 63), several of these genes exhibited depot-specific variability in expression which correlated closely with body mass index and/or waist-to-hip ratio. In conclusion, intrinsically programmed differences in gene expression patterns during adipogenesis suggest that fat depot specific regulation of adipogenesis contributes to individual risk of obesity.
- Andere Ausgabe
- Link zur Erstveröffentlichung
Link: https://doi.org/10.3389/fendo.2020.00138 - Freie Schlagwörter (EN)
- adipogenesis, transcriptome, adipocyte, mRNA, gene expression, TTCA
- Klassifikation (DDC)
- 610
- Verlag
- Frontiers Research Foundation, Lausanne
- Version / Begutachtungsstatus
- publizierte Version / Verlagsversion
- URN Qucosa
- urn:nbn:de:bsz:15-qucosa2-845537
- Veröffentlichungsdatum Qucosa
- 04.04.2023
- Dokumenttyp
- Artikel
- Sprache des Dokumentes
- Englisch
- Lizenz / Rechtehinweis
- CC BY 4.0