- AutorIn
- Yong Xu Technische Universität Dresden, B CUBE Center for Molecular Bioengineering
- Michelle Patino GaillezTechnische Universität Dresden, B CUBE Center for Molecular Bioengineering
- Kai Zheng
- Dagmar Voigt
- Meiying Cui
- Thomas Kurth
- Lingfei Xiao
- Robert Wieduwild
- Rebecca Rothe
- Sandra Hauser
- Pao-Wan Lee
- Weilin Lin
- Martin Bornhäuser
- Jens Pietzsch
- Aldo R. Boccaccini
- Yixin Zhang
- Titel
- A Self-Assembled Matrix System for Cell-Bioengineering Applications in Different Dimensions, Scales, and Geometries
- Zitierfähige Url:
- https://nbn-resolving.org/urn:nbn:de:bsz:14-qucosa2-891079
- Quellenangabe
- Small : nano micro
Erscheinungsjahr: 2022
Jahrgang: 18
Heft: 13
E-ISSN: 1613-6829
Artikelnummer: 2104758 - Erstveröffentlichung
- 2022
- Abstract (EN)
- Stem cell bioengineering and therapy require different model systems and materials in different stages of development. If a chemically defined biomatrix system can fulfill most tasks, it can minimize the discrepancy among various setups. By screening biomaterials synthesized through a coacervation-mediated self-assembling mechanism, a biomatrix system optimal for 2D human mesenchymal stromal cell (hMSC) culture and osteogenesis is identified. Its utility for hMSC bioengineering is further demonstrated in coating porous bioactive glass scaffolds and nanoparticle synthesis for esiRNA delivery to knock down the SOX-9 gene with high delivery efficiency. The self-assembled injectable system is further utilized for 3D cell culture, segregated co-culture of hMSC with human umbilical vein endothelial cells (HUVEC) as an angiogenesis model, and 3D bioprinting. Most interestingly, the coating of bioactive glass with the self-assembled biomatrix not only supports the proliferation and osteogenesis of hMSC in the 3D scaffold but also induces the amorphous bioactive glass (BG) scaffold surface to form new apatite crystals resembling bone-shaped plate structures. Thus, the self-assembled biomatrix system can be utilized in various dimensions, scales, and geometries for many different bioengineering applications.
- Andere Ausgabe
- Link zum Artikel, der zuerst in der Zeitschrift „Small : nano micro” erschienen ist.
DOI: 10.1002/smll.202104758 - Verweis
- Ergänzendes Material ist unter folgendem Link zu finden.
Link: https://onlinelibrary.wiley.com/doi/10.1002/smll.202104758#support-information-section - Freie Schlagwörter (DE)
- 3D-Druck, bioaktive Glasgerüste, Zell-Bio-Engineering, extrazelluläre Matrix, injizierbare Hydrogele, selbstorganisierte Matrix
- Freie Schlagwörter (EN)
- 3D printing, bioactive glass scaffolds, cell-bioengineering, extracellular matrix, injectable hydrogels, self-assembled matrix
- Klassifikation (DDC)
- 570
- 620
- Verlag
- Wiley-VCH, Weinheim
- Förder- / Projektangaben
- Bundesministerium für Bildung und Forschung (BMBF)
B CUBE Center for Molecular Bioengineering
ID: 03Z22E511 - Version / Begutachtungsstatus
- publizierte Version / Verlagsversion
- URN Qucosa
- urn:nbn:de:bsz:14-qucosa2-891079
- Veröffentlichungsdatum Qucosa
- 22.04.2024
- Dokumenttyp
- Artikel
- Sprache des Dokumentes
- Englisch
- Lizenz / Rechtehinweis
- CC BY 4.0