- AutorIn
- Joanne E. Adaway Department of Clinical Biochemistry, University Hospital South Manchester NHS Foundation Trust, Manchester, UK#Manchester Academic Health Science Centre, University Hospital South Manchester, The University of Manchester, Manchester, UK
- Mirko PeitzschTechnische Universität Dresden, Institute of Clinical Chemistry and Laboratory Medicine, Faculty Carl Gustav Carus
- Brian G. KeevilDepartment of Clinical Biochemistry, University Hospital South Manchester NHS Foundation Trust, Manchester, UK#Manchester Academic Health Science Centre, University Hospital South Manchester, The University of Manchester, Manchester, UK
- Titel
- A novel method for the measurement of plasma metanephrines using online solid phase extraction-liquid chromatography tandem mass spectrometry
- Zitierfähige Url:
- https://nbn-resolving.org/urn:nbn:de:bsz:14-qucosa2-354293
- Quellenangabe
- Annals of Clinical Biochemistry : International Journal of Biochemistry and Laboratory Medicine
Erscheinungsort: London
Verlag: Sage
Erscheinungsjahr: 2015
Jahrgang: 52
Heft: 3
Seiten: 361-369
E-ISSN: 1758-1001 - Erstveröffentlichung
- 2015
- Abstract (EN)
- Background: Measurement of plasma metanephrine, normetanephrine and 3-methoxytyramine is useful in the diagnosis of phaeochromocytomas, but many assays require a large volume of plasma due to poor assay sensitivity, and often require lengthy sample preparation. Our aim was to develop a method for measurement of plasma metanephrines using a small sample volume with minimal hands-on preparation. Methods: Samples were deproteinised using 10 K spin filters prior to online solid phase extraction using a Waters Acquity UPLC Online SPE Manager (Waters, Manchester, UK) coupled to a Waters Xevo TQ-S mass spectrometer (Waters, Manchester, UK). The assay was validated and results compared to a previously published method. Results: We achieved a limit of quantification of 37.5 pmol/L for metanephrine and 3-methoxytyramine and 75 pmol/L for normetanephrine using only 150 mL of sample. The assay was linear up to 30,000 pmol/L for all analytes and in a method comparison study results showed good agreement with a previously published LC-MS/MS assay. Conclusions: We have developed a simple method for measurement of plasma metanephrine, normetanephrine and 3-methoxytyramine using only 150 mL of sample. There is minimal hands-on sample preparation required and the assay is suitable for routine use in a clinical laboratory.
- Andere Ausgabe
- Link zum Artikel, der zuerst in der Zeitschrift 'Annals of Clinical Biochemistry' erschienen ist.
DOI: 10.1177/0004563214546560 - Freie Schlagwörter (DE)
- Metanephrin, Normetanephrin, 3-Methoxytyramin, Phäochromozytom, LC-MS/MS, Online-Festphasenextraktion
- Freie Schlagwörter (EN)
- Metanephrine, normetanephrine, 3-methoxytyramine, phaeochromocytoma, LC-MS/MS, online solid phase extraction
- Klassifikation (DDC)
- 610
- Verlag
- Sage, London
- Version / Begutachtungsstatus
- publizierte Version / Verlagsversion
- URN Qucosa
- urn:nbn:de:bsz:14-qucosa2-354293
- Veröffentlichungsdatum Qucosa
- 19.09.2019
- Dokumenttyp
- Artikel
- Sprache des Dokumentes
- Englisch
- Lizenz / Rechtehinweis