- AutorIn
- Dr. Astrid Schuller
- Gerhard Rödel
- Dr. Kai Ostermann
- Titel
- Tuning the Sensitivity of the PDR5 Promoter-Based Detection of Diclofenac in Yeast Biosensors
- Zitierfähige Url:
- https://nbn-resolving.org/urn:nbn:de:bsz:14-qucosa-230652
- Quellenangabe
- Sensors (2017), 17(7), ISSN 1424-8220. DOI: 10.3390/s17071506. Artikelnr.: 1506.
- Erstveröffentlichung
- 2017
- Abstract (DE)
- The commonly used drug diclofenac is an important environmental anthropogenic pollutant. Currently, detection of diclofenac is mainly based on chemical and physical methods. Here we describe a yeast biosensor that drives the diclofenac-dependent expression of a recombinant fluorescent protein from the authentic promoter of the PDR5 gene. This key component of the pleiotropic drug response encodes a multidrug transporter that is involved in cellular detoxification. We analyse the effects on diclofenac sensitivity of artificial PDR5 promoter derivatives in wild-type and various yeast mutant strains. This approach enabled us to generate sensor strains with elevated drug sensitivity.
- Andere Ausgabe
- DOI: 10.3390/s17071506
- Link zum Artikel, der zuerst in der Zeitschrift 'Sensors' erschienen ist.
Link: http://dx.doi.org/10.3390/s17071506 - Freie Schlagwörter (DE)
- Diclofenac, PDR5, Hefe, Biosensor, Technische Universität Dresden, Publikationsfonds
- Freie Schlagwörter (EN)
- diclofenac, PDR5, yeast, biosensor, Technische Universität Dresden, Publishing Fund
- Klassifikation (DDC)
- 620
- Klassifikation (RVK)
- ZI 0001
- Verlag
- MDPI (Multidisciplinary Digital Publishing Institute), Basel
- URN Qucosa
- urn:nbn:de:bsz:14-qucosa-230652
- Veröffentlichungsdatum Qucosa
- 15.11.2017
- Dokumenttyp
- Artikel
- Sprache des Dokumentes
- Englisch
- Lizenz / Rechtehinweis
- CC BY 4.0