Article
Is the intensity of the 5-ALA-derived fluorescence related to the light source?
Ist die Intensität der 5-ALA Fluoreszenz abhängig von der Lichtquelle?
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Published: | May 8, 2019 |
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Objective: Introduction of the 5-ALA technique is one major advance in surgical neuro-oncology. 5-ALA fluorescence guided resection of malignant glioma results in more complete surgical resections and subsequently prolonged survival. However, it remains questionable how light intensities of the blue light source and 5-ALA-derived fluorescence intensities of the illuminated tissue are connected. Aim of the present study was to compare light intensities of different blue light sources and Protoporphyrin (PpIX) fluorescence intensities of PpIX solutions with defined concentrations after illumination with different light sources.
Methods: Light spectrum of seven different blue light sources and fluorescence intensity of two PpIX solutions was quantified after illumination. The Zeiss OPMI Pentero microscope, the Zeiss OPMI Pentero 900 microscope, the Leica M530 OH6 microscope, an endoscope equipped with the 5-ALA technique, a mini-spectrometer equipped with a multi-channel LED source (MCLS) emitting monochromatic, a modified commercially available LED-Head Lamp and a commercially available unmodified UV-LED lamp were compared. PpIX was quantified in a standardized setup using a mini-spectrometer.
Results: Maximal light intensities of the evaluated light sources were reached at different wave lengths. All tested devices were sufficient to induce PpIX-induced fluorescence. However, intensity of PpIX-fluorescence of the 0.15 µg/ml and the 5 µg/ml was significantly related to the light sources.
Conclusion: Intensity of the 5-ALA-derived fluorescence is related to the light source used.