Soy supplementation: Impact on gene expression in different tissues of ovariectomized rats and evaluation of the rat model to predict (post)menopausal health effect.

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Version: Final published version
License: CC BY-NC-ND 4.0
Serval ID
serval:BIB_CB40B5F86381
Type
Article: article from journal or magazin.
Publication sub-type
Case report (case report): feedback on an observation with a short commentary.
Collection
Publications
Institution
Title
Soy supplementation: Impact on gene expression in different tissues of ovariectomized rats and evaluation of the rat model to predict (post)menopausal health effect.
Journal
Toxicology reports
Author(s)
Islam M.A., Hooiveld GJEJ, van den Berg JHJ, van der Velpen V., Murk A.J., Rietjens IMCM, van Leeuwen FXR
ISSN
2214-7500 (Electronic)
ISSN-L
2214-7500
Publication state
Published
Issued date
2018
Peer-reviewed
Oui
Volume
5
Pages
1087-1097
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Abstract
This toxicogenomic study was conducted to predict (post)menopausal human health effects of commercial soy supplementation using ovariectomized rats as a model. Different target tissues (i.e. breast, uterus and sternum) and non-target tissues (i.e. peripheral blood mononuclear cells (PBMC), adipose and liver) of ovariectomized F344 rats exposed to a commercially available soy supplement for eight weeks, were investigated. Changes in gene expression in these tissues were analysed using whole-genome microarray analysis. No correlation in changes in gene expression were observed among different tissues, indicating tissue specific effects of soy isoflavone supplementation. Out of 87 well-established estrogen responsive genes (ERGs), only 19 were found to be significantly regulated (p < 0.05) in different tissues, particularly in liver, adipose and uterus tissues. Surprisingly, no ERGs were significantly regulated in estrogen sensitive breast and sternum tissues. The changes in gene expression in PBMC and adipose tissue in rats were compared with those in (post)menopausal female volunteers who received the same supplement in a similar oral dose and exposure duration in human intervention studies. No correlation in changes in gene expression between rats and humans was observed. Although receiving a similar dose, in humans the plasma levels expressed as total free aglycones were several folds higher than in the rat. Therefore, the overall results in young ovariectomized female F344 rats indicated that using rat transcriptomic data does not provide a suitable model for human risk or benefit analysis of soy isoflavone supplementation.
Keywords
(Post)menopausal health effect, ERGs, estrogen responsive genes, ERα, estrogen receptor alpha, ERβ, estrogen receptor beta, Gene expression, Ovariectomized rat model, PBMC, peripheral blood mononuclear cells, Soy isoflavone supplementation
Pubmed
Open Access
Yes
Create date
20/11/2018 9:20
Last modification date
21/08/2019 7:10
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