Book/Dissertation / PhD Thesis

FZJ-2018-03414

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Control of neuron adhesion by metal nanoparticles

Tran, A. Q. (Corresponding author)FZJ*

2018
Forschungszentrum Jülich GmbH Zentralbibliothek, Verlag Jülich
ISBN: 978-3-95806-332-7

Please use a persistent id in citations: http://hdl.handle.net/2128/19397  urn:nbn:de:0001-2018080908

Abstract: The interaction between neurons and nanostructured materials is an increasing interest due to the possibility to manipulate the cells on the length scale of an individual biomolecule. A comprehensive understanding of neuron adhesion to non-biomaterials opens promising strategies to design neuronal network and for neuron-electrode integration. The neuronal adhesion can be mediated by specific receptor-ligand or non-specific interactions. The specific cell adhesion is often associated with the interactions between cell surface receptors (integrins)and their respective ligands from the extra cellular matrix (ECM) components or between hemophilic neuronal cell adhesion molecules (NCAMs) for cell-cell interactions. The nonspecific cell adhesion is mainly induced by electrostatic interactions. NCAMs are associated with the negatively charge polysialic acid (PSA) and are known to be crucial for regulating neuronal adhesion. Previously, the chemical ligand 11-amino-1-undecanethiol (AUT)possessing positively charged terminals have been used to functionalize gold nanoparticles(AuNPs) on the substrate for tuning the neuron adhesion and neurite outgrowth. However, the preparation of cell culture samples has been restricted to the immobilization of disordered AuNPs only. Moreover, it has been found that the attachment of these AuNPs on the surface has been instable during long time cell culture, which results in particle aggregation and cytotoxicity due to particle uptake. Although the cell adhesion is obviously mediated by the electrostatic interactions, further studies to understand how charges and mechanical properties of the substrates affect the cell adhesion and neurite outgrowth are still missing. In this work, the block copolymer micelle nanolithography is used to synthesize both ordered and disordered AuNP arrays of different sizes and densities. Moreover, weakly bound (WB) and strongly bound(SB) AuNPs on the substrates can be obtained by tuning the oxygen plasma exposure time. The AuNPs are used as nanoplatforms to carry the AUT ligands, while the background is blocked by cell aversive molecules of 2-[methoxyl(polyethyleneoxy)6-9-propyl]trichlosilane (PEG). [...]

Note: RWTH Aachen, Diss., 2018

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Contributing Institute(s):

1. Bioelektronik (ICS-8)

Research Program(s):

1. 552 - Engineering Cell Function (POF3-552) (POF3-552)

Appears in the scientific report 2018

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