- AutorIn
- Claudia D. Deumer
- Titel
- RNA-binding proteins in yeast mitochondria
- Zitierfähige Url:
- https://nbn-resolving.org/urn:nbn:de:swb:14-1035897639531-83407
- Übersetzter Titel (DE)
- RNA-bindende Proteine in Hefemitochondrien
- Datum der Einreichung
- 12.07.2002
- Datum der Verteidigung
- 09.10.2002
- Abstract (EN)
- This work focused on the further characterisation of Idhp and of the Krebs cycle enzymes citrate synthase 1 (Cit1p) and malate dehydrogenase 1 (Mdh1p) both of which have been identified as RNA-binding proteins without known RNA recognition motifs. Besides analysing their effects on mitochondrial translation and their organisation in protein complexes the work focused on the characterisation of the RNA-binding properties of recombinant Cit1p and Mdh1p: · Cit1p and Mdh1p play no essential role in mitochondrial protein synthesis. · Idhp is in a complex of molecular weight larger than the cytochrome c oxidase (250 kDa). · Cit1p and Mdh1p are in mitochondrial complexes smaller than 250 kDa. · 1000-fold molar excess of tRNA referring to COX2 leader RNA did not inhibit the RNA-binding of Cit1p and Mdh1p. · Cit1p and Mdh1p bind mitochondrial mRNAs (sense and antisense). The influence of cofactors and substrates on RNA-binding was analysed in order to reveal a possible link between the enzymatic function and the property of RNA-binding: · Acetyl-CoA and ATP inhibited the RNA-binding of Cit1p and Mdh1p at a concentration of 5 mM.
- Freie Schlagwörter (DE)
- Krebszyklusenzyme, RNA-Bindung, mitochondriale Translation
- Freie Schlagwörter (EN)
- Krebs cycle enzymes, RNA-binding, mitochondrial translation
- Klassifikation (DDC)
- 32
- Klassifikation (RVK)
- WG 1890
- Normschlagwörter (GND)
- Citronensäurezyklus, Enzym, Hefeartige Pilze, Mitochondrium, RNS-Bindungsproteine, Translation
- GutachterIn
- Prof. Dr. Gerhard Rödel
- Prof. Dr. Leslie A. Grivell
- Prof. Dr. Michael Göttfert
- BetreuerIn
- Prof. Dr. Gerhard Rödel
- Verlag
- Technische Universität Dresden, Dresden
- URN Qucosa
- urn:nbn:de:swb:14-1035897639531-83407
- Veröffentlichungsdatum Qucosa
- 06.12.2002
- Dokumenttyp
- Dissertation
- Sprache des Dokumentes
- Englisch
- Lizenz / Rechtehinweis