Biomineralization of engineered spider silk protein-based composite materials for bone tissue engineering

Materials based on biodegradable polyesters, such as poly(butylene terephthalate) (PBT) or poly(butylene terephthalate-co-poly(alkylene glycol) terephthalate) (PBTAT), have potential application as pro-regenerative scaffolds for bone tissue engineering. Herein, the preparation of films composed of PBT or PBTAT and an engineered spider silk protein, (eADF4(C16)), that displays multiple carboxylic acid moieties capable of binding calcium ions and facilitating their biomineralization with calcium carbonate or calcium phosphate is reported. Human mesenchymal stem cells cultured on films mineralized with calcium phosphate show enhanced levels of alkaline phosphatase activity suggesting that such composites have potential use for bone tissue engineering.

Metadaten
Author details:	John G. Hardy, Jose Guillermo Torres-Rendon, Aldo Leal-Egaña, Andreas Walther, Helmut Schlaad ORCiD GND, Helmut Cölfen GND, Thomas R. Scheibel ORCiD GND
URN:	urn:nbn:de:kobv:517-opus4-400519
Publication series (Volume number):	Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe (359)
Publication type:	Postprint
Language:	English
Date of first publication:	2017/09/22
Publication year:	2017
Publishing institution:	Universität Potsdam
Release date:	2017/09/22
Tag:	biodegradable polymers; biomaterials; biomineralization; bone tissue engineering; recombinant protein; spider silk
Number of pages:	13
Source:	Materials 9 (2016) Nr. 7. - DOI: 10.3390/ma9070560
Organizational units:	Mathematisch-Naturwissenschaftliche Fakultät / Institut für Chemie
DDC classification:	6 Technik, Medizin, angewandte Wissenschaften / 60 Technik / 600 Technik, Technologie
Peer review:	Referiert
Publishing method:	Open Access
Grantor:	Multidisciplinary Digital Publishing Institute (MDPI)
License (German):	CC-BY - Namensnennung 4.0 International