Structural insights into the catalytic binuclear center of cytochrome c oxidase from Paracoccus denitrificans
- Cytochrome c oxidases are among the most important and fundamental enzymes of life. Integrated into membranes they use four electrons from cytochrome c molecules to reduce molecular oxygen (dioxygen) to water. Their catalytic cycle has been considered to start with the oxidized form. Subsequent electron transfers lead to the E-state, the R-state (which binds oxygen), the P-state (with an already split dioxygen bond), the F-state and the O-state again. Here, we determined structures of up to 1.9 Å resolution of these intermediates by single particle cryo-EM. Our results suggest that in the O-state the active site contains a peroxide dianion and in the P-state possibly an intact dioxygen molecule, the F-state may contain a superoxide anion.
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| Author: | Felix Martin Kolbe |
|---|---|
| URN: | urn:nbn:de:hebis:30:3-669553 |
| DOI: | https://doi.org/10.21248/gups.66955 |
| Place of publication: | Frankfurt am Main |
| Referee: | Clemens GlaubitzORCiDGND, Hartmut MichelORCiDGND |
| Advisor: | Hartmut Michel |
| Document Type: | Doctoral Thesis |
| Language: | English |
| Date of Publication (online): | 2022/02/22 |
| Year of first Publication: | 2021 |
| Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
| Granting Institution: | Johann Wolfgang Goethe-Universität |
| Date of final exam: | 2022/02/21 |
| Release Date: | 2022/03/16 |
| Page Number: | 109 |
| HeBIS-PPN: | 491802234 |
| Institutes: | Biochemie, Chemie und Pharmazie |
| Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
| Sammlungen: | Universitätspublikationen |
| Licence (German): |  Deutsches Urheberrecht |
