Complexome profiling: assembly and remodeling of protein complexes
- Many proteins have been found to operate in a complex with various biomolecules such as proteins, nucleic acids, carbohydrates, or lipids. Protein complexes can be transient, stable or dynamic and their association is controlled under variable cellular conditions. Complexome profiling is a recently developed mass spectrometry-based method that combines mild separation techniques, native gel electrophoresis, and density gradient centrifugation with quantitative mass spectrometry to generate inventories of protein assemblies within a cell or subcellular fraction. This review summarizes applications of complexome profiling with respect to assembly ranging from single subunits to large macromolecular complexes, as well as their stability, and remodeling in health and disease.
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| Author: | Ilka WittigORCiD, Pedro Felipe MalacarneORCiDGND |
|---|---|
| URN: | urn:nbn:de:hebis:30:3-618958 |
| DOI: | https://doi.org/10.3390/ijms22157809 |
| ISSN: | 1422-0067 |
| Parent Title (English): | International journal of molecular sciences |
| Publisher: | MDPI |
| Place of publication: | Basel |
| Document Type: | Article |
| Language: | English |
| Date of Publication (online): | 2021/07/21 |
| Date of first Publication: | 2021/07/21 |
| Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
| Release Date: | 2021/07/30 |
| Tag: | assembly; complexome profiling; data repositories; mass spectrometry; protein complexes; protein–protein interaction; remodeling |
| Volume: | 22 |
| Issue: | 15, art. 7809 |
| Page Number: | 12 |
| First Page: | 1 |
| Last Page: | 12 |
| HeBIS-PPN: | 487211847 |
| Institutes: | Medizin |
| Dewey Decimal Classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
| Sammlungen: | Universitätspublikationen |
| Open-Access-Publikationsfonds: | Medizin |
| Licence (German): |  Creative Commons - Namensnennung 4.0 |
