Toxicological Characterization of the Inorganic and Organic Arsenic Metabolite Thio-DMAV in Cultured Human Lung Cells
We synthesised and toxicologically characterised the arsenic metabolite thiodimethylarsinic acid (thio-DMAV). Successful synthesis of highly pure thio-DMAV was confirmed by state-of-the-art analytical techniques including 1H-NMR, HPLC-FTMS, and HPLC-ICPMS. Toxicological characterization was carried...
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FB/Einrichtung: | FB 12: Chemie und Pharmazie |
Dokumenttypen: | Artikel |
Medientypen: | Text |
Erscheinungsdatum: | 2011 |
Publikation in MIAMI: | 20.02.2013 |
Datum der letzten Änderung: | 16.04.2019 |
Angaben zur Ausgabe: | [Electronic ed.] |
Quelle: | Journal of Toxicology 2011 (2011) 373141 |
Fachgebiet (DDC): | 540: Chemie |
Lizenz: | CC BY 2.5 |
Sprache: | English |
Anmerkungen: | Finanziert durch den Open-Access-Publikationsfonds 2011/2012 der Deutschen Forschungsgemeinschaft (DFG) und der Westfälischen Wilhelms-Universität Münster (WWU Münster). |
Format: | PDF-Dokument |
URN: | urn:nbn:de:hbz:6-87379477683 |
Weitere Identifikatoren: | DOI: 10.1155/2011/37314 |
Permalink: | https://nbn-resolving.de/urn:nbn:de:hbz:6-87379477683 |
Onlinezugriff: | 373141.pdf |
We synthesised and toxicologically characterised the arsenic metabolite thiodimethylarsinic acid (thio-DMAV). Successful synthesis of highly pure thio-DMAV was confirmed by state-of-the-art analytical techniques including 1H-NMR, HPLC-FTMS, and HPLC-ICPMS. Toxicological characterization was carried out in comparison to arsenite and its well-known trivalent and pentavalent methylated metabolites. It comprised cellular bioavailability as well as different cytotoxicity and genotoxicity end points in cultured human A549 lung cells. Of all arsenicals investigated, thio-DMAV exerted the strongest cytotoxicity. Moreover, thio-DMAV did not induce DNA strand breaks and an increased induction of both micronuclei and multinucleated cells occurred only at beginning cytotoxic concentrations, indicating that thio-DMAV does not act via a genotoxic mode of action. Finally, to assess potential implications of thio-DMAV for human health, further mechanistic studies are urgently necessary to identify the toxic mode of action of this highly toxic, unusual pentavalent organic arsenical.