Physicochemical and biological characterization of chitosan-microRNA nanocomplexes for gene delivery to MCF-7 breast cancer cells
Cancer gene therapy requires the design of non-viral vectors that carry genetic material and selectively deliver it with minimal toxicity. Non-viral vectors based on cationic natural polymers can form electrostatic complexes with negatively-charged polynucleotides such as microRNAs (miRNAs). Here we...
| Verfasser: | |
|---|---|
| FB/Einrichtung: | FB 13: Biologie |
| Dokumenttypen: | Artikel |
| Medientypen: | Text |
| Erscheinungsdatum: | 2015 |
| Publikation in MIAMI: | 08.10.2015 |
| Datum der letzten Änderung: | 18.07.2022 |
| Angaben zur Ausgabe: | [Electronic ed.] |
| Quelle: | Scientific Reports 5 (2015) 13567, 1-15 |
| Fachgebiet (DDC): | 570: Biowissenschaften; Biologie |
| Lizenz: | CC BY 4.0 |
| Sprache: | English |
| Anmerkungen: | Finanziert durch den Open-Access-Publikationsfonds 2015/2016 der Westfälischen Wilhelms-Universität Münster (WWU Münster). |
| Format: | PDF-Dokument |
| ISSN: | 2045-2322 |
| URN: | urn:nbn:de:hbz:6-78219455692 |
| Weitere Identifikatoren: | DOI: 10.1038/srep13567 |
| Permalink: | https://nbn-resolving.de/urn:nbn:de:hbz:6-78219455692 |
| Onlinezugriff: | srep13567.pdf |
Cancer gene therapy requires the design of non-viral vectors that carry genetic material and selectively deliver it with minimal toxicity. Non-viral vectors based on cationic natural polymers can form electrostatic complexes with negatively-charged polynucleotides such as microRNAs (miRNAs). Here we investigated the physicochemical/biophysical properties of chitosan–hsa-miRNA-145 (CS–miRNA) nanocomplexes and the biological responses of MCF-7 breast cancer cells cultured in vitro. Self-assembled CS–miRNA nanocomplexes were produced with a range of (+/−) charge ratios (from 0.6 to 8) using chitosans with various degrees of acetylation and molecular weight. The Z-average particle diameter of the complexes was