Bilateral inflammation after unilateral UVR-B irradiationOcular cross-talk and immunomodulation between exposed and unexposed, contralateral eye
Bilateral inflammation after unilateral UVR-B irradiation
Ocular cross-talk and immunomodulation between exposed and unexposed, contralateral eye
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DissertationDate of Exam
18.12.2020Date of Publication
29.01.2021Advisor
Wegener, Alfred R.Co-Referee
Latz, EickeMetadata
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Abstract
The presented study investigates the influence of exposure of one eye to UVR-B on the unexposed, partner eye in vivo in a mouse model. Furthermore, we try to answer the question whether this sympathizing reaction of the partner eye is transmitted via a neurokinin-dependent signaling pathway of substance P (SP) and its neurokinin-1 receptor (NKR-1). In all experiments mice were unilaterally exposed in vivo to UVR-B. The unexposed eyes were completely shielded during exposure time. NKR-1 protein levels in ocular tissues were quantified by immunohistochemical staining and enzyme-linked immunosorbent assay (ELISA). In a second experiment, NKR-1 was blocked with NKR-1 antagonists. Ocular tissues were extracted for the detection of pro-inflammatory cytokine and chemokine expression by ProcartaPlexTM Multiplex Immunoassay. The UVR-B radiation induces the expression and upregulates NKR-1 in the exposed and unexposed, partner eye. Pretreatment with Fosaprepitant decreased NKR-1 expression in all exposed ocular tissues as well as in the unexposed lens epithelium. Overall, UVR-B exposure triggers NKR-1 upregulation not only in the exposed but also in the partner eye in various ocular tissues. In addition, the clinical approved NKR-1 antagonist Fosaprepitant effectively reduces NKR-1 expression in both mouse eyes.
Subjects
Katarakt, Substanz P, UVB Strahlung, Fosaprepitant, Cataract, Substance P, UVR-B irradiation
Classification (DDC)
570 Biowissenschaften, Biologie 610 Medizin, GesundheitMarx, Janine: Bilateral inflammation after unilateral UVR-B irradiation : Ocular cross-talk and immunomodulation between exposed and unexposed, contralateral eye. - Bonn, 2021. - Dissertation, Rheinische Friedrich-Wilhelms-Universität Bonn.
Online-Ausgabe in bonndoc: https://nbn-resolving.org/urn:nbn:de:hbz:5-60910
Online-Ausgabe in bonndoc: https://nbn-resolving.org/urn:nbn:de:hbz:5-60910
@phdthesis{handle:20.500.11811/8905,
urn: https://nbn-resolving.org/urn:nbn:de:hbz:5-60910,
author = {{Janine Marx}},
title = {Bilateral inflammation after unilateral UVR-B irradiation : Ocular cross-talk and immunomodulation between exposed and unexposed, contralateral eye},
school = {Rheinische Friedrich-Wilhelms-Universität Bonn},
year = 2021,
month = jan,
note = {The presented study investigates the influence of exposure of one eye to UVR-B on the unexposed, partner eye in vivo in a mouse model. Furthermore, we try to answer the question whether this sympathizing reaction of the partner eye is transmitted via a neurokinin-dependent signaling pathway of substance P (SP) and its neurokinin-1 receptor (NKR-1). In all experiments mice were unilaterally exposed in vivo to UVR-B. The unexposed eyes were completely shielded during exposure time. NKR-1 protein levels in ocular tissues were quantified by immunohistochemical staining and enzyme-linked immunosorbent assay (ELISA). In a second experiment, NKR-1 was blocked with NKR-1 antagonists. Ocular tissues were extracted for the detection of pro-inflammatory cytokine and chemokine expression by ProcartaPlexTM Multiplex Immunoassay. The UVR-B radiation induces the expression and upregulates NKR-1 in the exposed and unexposed, partner eye. Pretreatment with Fosaprepitant decreased NKR-1 expression in all exposed ocular tissues as well as in the unexposed lens epithelium. Overall, UVR-B exposure triggers NKR-1 upregulation not only in the exposed but also in the partner eye in various ocular tissues. In addition, the clinical approved NKR-1 antagonist Fosaprepitant effectively reduces NKR-1 expression in both mouse eyes.},
url = {https://hdl.handle.net/20.500.11811/8905}
}
urn: https://nbn-resolving.org/urn:nbn:de:hbz:5-60910,
author = {{Janine Marx}},
title = {Bilateral inflammation after unilateral UVR-B irradiation : Ocular cross-talk and immunomodulation between exposed and unexposed, contralateral eye},
school = {Rheinische Friedrich-Wilhelms-Universität Bonn},
year = 2021,
month = jan,
note = {The presented study investigates the influence of exposure of one eye to UVR-B on the unexposed, partner eye in vivo in a mouse model. Furthermore, we try to answer the question whether this sympathizing reaction of the partner eye is transmitted via a neurokinin-dependent signaling pathway of substance P (SP) and its neurokinin-1 receptor (NKR-1). In all experiments mice were unilaterally exposed in vivo to UVR-B. The unexposed eyes were completely shielded during exposure time. NKR-1 protein levels in ocular tissues were quantified by immunohistochemical staining and enzyme-linked immunosorbent assay (ELISA). In a second experiment, NKR-1 was blocked with NKR-1 antagonists. Ocular tissues were extracted for the detection of pro-inflammatory cytokine and chemokine expression by ProcartaPlexTM Multiplex Immunoassay. The UVR-B radiation induces the expression and upregulates NKR-1 in the exposed and unexposed, partner eye. Pretreatment with Fosaprepitant decreased NKR-1 expression in all exposed ocular tissues as well as in the unexposed lens epithelium. Overall, UVR-B exposure triggers NKR-1 upregulation not only in the exposed but also in the partner eye in various ocular tissues. In addition, the clinical approved NKR-1 antagonist Fosaprepitant effectively reduces NKR-1 expression in both mouse eyes.},
url = {https://hdl.handle.net/20.500.11811/8905}
}
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