Impact of ROS-Induced Damage of TCA Cycle Enzymes on Metabolism and Virulence of Salmonella enterica serovar Typhimurium
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https://osnadocs.ub.uni-osnabrueck.de/handle/urn:nbn:de:gbv:700-201907171818
https://osnadocs.ub.uni-osnabrueck.de/handle/urn:nbn:de:gbv:700-201907171818
Titel: | Impact of ROS-Induced Damage of TCA Cycle Enzymes on Metabolism and Virulence of Salmonella enterica serovar Typhimurium |
Autor(en): | Noster, Janina Persicke, Marcus Chao, Tzu-Chiao Krone, Lena Heppner, Bianca Hensel, Michael Hansmeier, Nicole |
ORCID des Autors: | https://orcid.org/0000-0001-6239-8614 https://orcid.org/0000-0002-8802-6605 https://orcid.org/0000-0001-8781-6958 https://orcid.org/0000-0001-6604-6253 |
Zusammenfassung: | Salmonella enterica serovar Typhimurium (STM) is exposed to reactive oxygen species (ROS) originating from aerobic respiration, antibiotic treatment, and the oxidative burst occurring inside the Salmonella-containing vacuole (SCV) within host cells. ROS damage cellular compounds, thereby impairing bacterial viability and inducing cell death. Proteins containing iron–sulfur (Fe–S) clusters are particularly sensitive and become non-functional upon oxidation. Comprising five enzymes with Fe–S clusters, the TCA cycle is a pathway most sensitive toward ROS. To test the impact of ROS-mediated metabolic perturbations on bacterial physiology, we analyzed the proteomic and metabolic profile of STM deficient in both cytosolic superoxide dismutases (ΔsodAB). Incapable of detoxifying superoxide anions (SOA), endogenously generated SOA accumulate during growth. ΔsodAB showed reduced abundance of aconitases, leading to a metabolic profile similar to that of an aconitase-deficient strain (ΔacnAB). Furthermore, we determined a decreased expression of acnA in STM ΔsodAB. While intracellular proliferation in RAW264.7 macrophages and survival of methyl viologen treatment were not reduced for STM ΔacnAB, proteomic profiling revealed enhanced stress response. We conclude that ROS-mediated reduced expression and damage of aconitase does not impair bacterial viability or virulence, but might increase ROS amounts in STM, which reinforces the bactericidal effects of antibiotic treatment and immune responses of the host. |
Bibliografische Angaben: | Front. Microbiol. 10:762, 2019 |
URL: | https://osnadocs.ub.uni-osnabrueck.de/handle/urn:nbn:de:gbv:700-201907171818 |
Schlagworte: | metabolomics; oxidative stress; iron–sulfur cluster damage; aconitase; superoxide dismutase |
Erscheinungsdatum: | 24-Apr-2019 |
Lizenzbezeichnung: | Attribution 4.0 International |
URL der Lizenz: | http://creativecommons.org/licenses/by/4.0/ |
Publikationstyp: | Einzelbeitrag in einer wissenschaftlichen Zeitschrift [article] |
Enthalten in den Sammlungen: | FB05 - Hochschulschriften Open-Access-Publikationsfonds |
Dateien zu dieser Ressource:
Datei | Beschreibung | Größe | Format | |
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fmicb_10_00762_201_Noster.pdf | 2,32 MB | Adobe PDF | fmicb_10_00762_201_Noster.pdf Öffnen/Anzeigen |
Diese Ressource wurde unter folgender Copyright-Bestimmung veröffentlicht: Lizenz von Creative Commons