Der Einfluss von FINEair® bei der Trocknung von Wurzelkanälen auf die Eindringtiefe von AH Temp™ in die Dentintubuli humaner Zähne

Abstract

Summary Background and objectives Root canal dressings are used to reduce the number of bacteria in infected root canal systems. For disinfection, the medicament must come into contact with the microorganisms and thus be able to penetrate the dentinal tubules. Appropriate drying of the root canals can increase the depth of penetration of materials into the dentinal tubules. The aim of this study is to investigate the influence of additional drying by using compressed air by FINEair® (KaVo, Biberach, Germany) on the penetration depth of the medicament AH Temp™ (Dentsply Detrey, Konstanz, Germany) into the dentinal tubules. Methods 40 human single-rooted teeth were collected and stored in chloramine T solution (1%) until usage. After decapitation, the roots were randomized into 4 groups of 10 teeth each. Prior to preparation with R50 Reciproc® files (VDW, Munich, Germany), a glide path of size ISO 20 had been created. During preparation of the root canal, it was rinsed alternately with sodium hypochlorite solution (3%) and citric acid (40%). In the final protocol sodium hypochlorite (3%) was passively sound-activated. No citric acid was used in the negative control group. The canals were dried with paper points and ethanol (96%) in group 1 and in the control groups. Group 2 was dried additionally with FINEair®. The rhodamine B-stained medicament AH Temp™ was used as medication for groups 1 and 2. The roots of the control groups were filled with rhodamine B dissolved in ethanol (Sigma Chemical Company, St. Louis, Missouri, USA). All roots were split into three parts and embedded in Technovit® (Heraeus Kulzer, Wehrheim, Germany). After gradually polishing, the samples were examined in a confocal laser-scanning microscope (CLSM) and the depth of penetration of AH Temp™ into the dentinal tubules was measured. Results The penetrations of AH Temp™ were compared both between the three root sections within a group and between the four groups. For absolute penetration, the total area of penetration was measured, for relative penetration, it was set in relation to the total area and for distance measurement it was measured at 8 fixed points. There were significant differences in the penetration of AH Temp™ into the dentinal tubules for almost all pairings of the apical, middle and coronal root sections with an alpha error probability of 5 percent. The pairings of the coronal and middle sections were exceptions regarding the relative penetration and the distance measurement of the absolute penetration. In general, the penetration depths decreased from coronal to apical. The lowest penetration depths were measured in the apical third of the negative control group at an average of 230 μm and the highest results were measured in the coronal third of the positive control group at an average of 1072 μm. There were no significant differences in the depth of penetration of AH Temp™ when comparing the four experimental groups among each other, with one exception. Only the relative penetration of group 2 with FINEair® was significantly lower than the one of the positive control group. Conclusion Additional drying of the root canals with FINEair® does not increase the depth of penetration of AH Temp™ into the dentinal tubules compared to drying with paper points and ethanol alone. For the clinical situation, this means that the use of FINEair® has no positive effect on the penetration depth of AH Temp™ and should be avoided due to possible complications. Show more