Prophylactic trypsin inhibition during the isolation procedure guarantees reproducible, high porcine islet yields

Please always quote using this URN: urn:nbn:de:bvb:20-opus-45531
  • Abstract: For isolating islets from the porcine pancreas, we established a semiautomated digestion method. Although the isolation technique was standardized and collagenase of controlled quality was used, until now the reproducibility of high islet yields was unsatisfactory. Our hypothesis was that pancreatic trypsin was responsible for this failure. The aim of this study was to investigate the effect of endogenous trypsin on islet yield. Our results demonstrate that a high trypsin level correlates with poor islet yield, whereas low trypsinAbstract: For isolating islets from the porcine pancreas, we established a semiautomated digestion method. Although the isolation technique was standardized and collagenase of controlled quality was used, until now the reproducibility of high islet yields was unsatisfactory. Our hypothesis was that pancreatic trypsin was responsible for this failure. The aim of this study was to investigate the effect of endogenous trypsin on islet yield. Our results demonstrate that a high trypsin level correlates with poor islet yield, whereas low trypsin activity always correlates with high islet yield. Specific inhibition of trypsin results in low trypsin activity and reproducible, high islet yields.show moreshow less

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Metadaten
Author: A. Heiser, Karin Ulrichs, W. Müller-Ruchholtz
URN:urn:nbn:de:bvb:20-opus-45531
Document Type:Journal article
Faculties:Medizinische Fakultät / Klinik und Poliklinik für Allgemein-, Viszeral-, Gefäß- und Kinderchirurgie (Chirurgische Klinik I)
Language:English
Year of Completion:1994
Source:In: Xenotransplantation (1994) 1, 66-68
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Tag:enzyme activation; enzyme inhibitors; islets of Langerhans; swine; xenogenic transplantation
Release Date:2010/09/03
Licence (German):License LogoDeutsches Urheberrecht