A duplicated copy of id2b is an unusual sex-determining candidate gene on the Y chromosome of arapaima (Arapaima gigas)

Please always quote using this URN: urn:nbn:de:bvb:20-opus-265672
  • Arapaima gigas is one of the largest freshwater fish species of high ecological and economic importance. Overfishing and habitat destruction are severe threats to the remaining wild populations. By incorporating a chromosomal Hi-C contact map, we improved the arapaima genome assembly to chromosome-level, revealing an unexpected high degree of chromosome rearrangements during evolution of the bonytongues (Osteoglossiformes). Combining this new assembly with pool-sequencing of male and female genomes, we identified id2bbY, a duplicated copy ofArapaima gigas is one of the largest freshwater fish species of high ecological and economic importance. Overfishing and habitat destruction are severe threats to the remaining wild populations. By incorporating a chromosomal Hi-C contact map, we improved the arapaima genome assembly to chromosome-level, revealing an unexpected high degree of chromosome rearrangements during evolution of the bonytongues (Osteoglossiformes). Combining this new assembly with pool-sequencing of male and female genomes, we identified id2bbY, a duplicated copy of the inhibitor of DNA binding 2b (id2b) gene on the Y chromosome as candidate male sex-determining gene. A PCR-test for id2bbY was developed, demonstrating that this gene is a reliable male-specific marker for genotyping. Expression analyses showed that this gene is expressed in juvenile male gonads. Its paralog, id2ba, exhibits a male-biased expression in immature gonads. Transcriptome analyses and protein structure predictions confirm id2bbY as a prime candidate for the master sex-determiner. Acting through the TGF beta signaling pathway, id2bbY from arapaima would provide the first evidence for a link of this family of transcriptional regulators to sex determination. Our study broadens our current understanding about the evolution of sex determination genetic networks and provide a tool for improving arapaima aquaculture for commercial and conservation purposes.show moreshow less

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Author: Mateus C. Adolfi, Kang Du, Susanne Kneitz, Cédric Cabau, Margot Zahm, Christophe Klopp, Romain Feron, Rômulo V. Paixão, Eduardo S. Varela, Fernanda L. de Almeida, Marcos A. de Oliveira, Rafael H. Nóbrega, Céline Lopez-Roques, Carole Iampietro, Jérôme Lluch, Werner Kloas, Sven Wuertz, Fabian Schaefer, Matthias Stöck, Yann Guiguen, Manfred Schartl
URN:urn:nbn:de:bvb:20-opus-265672
Document Type:Journal article
Faculties:Medizinische Fakultät / Theodor-Boveri-Institut für Biowissenschaften
Medizinische Fakultät / Comprehensive Cancer Center Mainfranken
Language:English
Parent Title (English):Scientific Reports
Year of Completion:2021
Volume:11
Issue:1
Article Number:21544
Source:Scientific Reports (2021) 11:1, 21544. https://doi.org/10.1038/s41598-021-01066-z
DOI:https://doi.org/10.1038/s41598-021-01066-z
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Tag:evolutionary genetics; genetic markers; genome
Release Date:2022/05/02
Open-Access-Publikationsfonds / Förderzeitraum 2021
Licence (German):License LogoCC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International