In vitro evaluation of antibacterial efficacy of vancomycin-loaded suture tapes and cerclage wires

Please always quote using this URN: urn:nbn:de:bvb:20-opus-260089
  • Usage of implants containing antibiotic agents has been a common strategy to prevent implant related infections in orthopedic surgery. Unfortunately, most implants with microbial repellent properties are characterized by accessibility limitations during daily clinical practice. Aim of this in vitro study was to investigate whether suture tapes and cerclage wires, which were treated with vancomycin, show a sustainable antibacterial activity. For this purpose, we used 24 stainless steel wire cerclages and 24 ultra-high molecular weightUsage of implants containing antibiotic agents has been a common strategy to prevent implant related infections in orthopedic surgery. Unfortunately, most implants with microbial repellent properties are characterized by accessibility limitations during daily clinical practice. Aim of this in vitro study was to investigate whether suture tapes and cerclage wires, which were treated with vancomycin, show a sustainable antibacterial activity. For this purpose, we used 24 stainless steel wire cerclages and 24 ultra-high molecular weight polyethylene and polyester suture tape test bodies. The test bodies were incubated for 30 min. in 100 mg/ml vancomycin solution or equivalent volumes of 0.9% NaCl. After measuring the initial solution uptake of the test bodies, antibacterial efficacy via agar diffusion test with Staphylococcus aureus and vancomycin elution tests were performed 1, 2, 3, and 6 days after incubation. Vancomycin-loaded tapes as well as vancomycin-loaded cerclage wires demonstrated increased bacterial growth inhibition when compared to NaCl-treated controls. Vancomycin-loaded tapes showed an additional twofold and eightfold increase of bacterial growth inhibition compared to vancomycin-loaded wires at day 1 and 2, respectively. Elution tests at day 1 revealed high levels of vancomycin concentration in vancomycin loaded tapes and wires. Additionally, the concentration in vancomycin loaded tapes was 14-fold higher when compared to vancomycin loaded wires. Incubating suture tapes and cerclage wires in vancomycin solution showed a good short-term antibacterial activity compared to controls. Considering the ease of vancomycin application on suture tapes or wires, our method could represent an attractive therapeutic strategy in biofilm prevention in orthopedic surgery.show moreshow less

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Metadaten
Author: Annette Eidmann, Andrea Ewald, Sebastian P. Boelch, Maximilian Rudert, Boris M. Holzapfel, Ioannis Stratos
URN:urn:nbn:de:bvb:20-opus-260089
Document Type:Journal article
Faculties:Medizinische Fakultät / Lehrstuhl für Orthopädie
Medizinische Fakultät / Abteilung für Funktionswerkstoffe der Medizin und der Zahnheilkunde
Language:English
Parent Title (English):Journal of Materials Science: Materials in Medicine
Year of Completion:2021
Volume:32
Issue:4
Pagenumber:42
Source:Journal of Materials Science: Materials in Medicine (2021) 32:42. doi: 10.1007/s10856-021-06513-x
DOI:https://doi.org/10.1007/s10856-021-06513-x
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 617 Chirurgie und verwandte medizinische Fachrichtungen
Tag:Staphylococcus aureus / drug effects; Vancomycin / administration & dosage; Vancomycin / chemistry; Vancomycin / pharmacology; anti-bacterial agents / administration & dosage; anti-bacterial agents / chemistry; anti-bacterial agents / pharmacology; biocompatible Materials; bone wires; drug liberation; materials testing; prostheses and implants; sutures
Release Date:2022/03/26
Open-Access-Publikationsfonds / Förderzeitraum 2021
Licence (German):License LogoCC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International