Metabolite ratios as quality indicators for pre-analytical variation in serum and EDTA plasma

Please always quote using this URN: urn:nbn:de:bvb:20-opus-246261
  • In clinical diagnostics and research, blood samples are one of the most frequently used materials. Nevertheless, exploring the chemical composition of human plasma and serum is challenging due to the highly dynamic influence of pre-analytical variation. A prominent example is the variability in pre-centrifugation delay (time-to-centrifugation; TTC). Quality indicators (QI) reflecting sample TTC are of utmost importance in assessing sample history and resulting sample quality, which is essential for accurate diagnostics and conclusive,In clinical diagnostics and research, blood samples are one of the most frequently used materials. Nevertheless, exploring the chemical composition of human plasma and serum is challenging due to the highly dynamic influence of pre-analytical variation. A prominent example is the variability in pre-centrifugation delay (time-to-centrifugation; TTC). Quality indicators (QI) reflecting sample TTC are of utmost importance in assessing sample history and resulting sample quality, which is essential for accurate diagnostics and conclusive, reproducible research. In the present study, we subjected human blood to varying TTCs at room temperature prior to processing for plasma or serum preparation. Potential sample QIs were identified by Ultra high pressure liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) based metabolite profiling in samples from healthy volunteers (n = 10). Selected QIs were validated by a targeted MS/MS approach in two independent sets of samples from patients (n = 40 and n = 70). In serum, the hypoxanthine/guanosine (HG) and hypoxanthine/inosine (HI) ratios demonstrated high diagnostic performance (Sensitivity/Specificity > 80%) for the discrimination of samples with a TTC > 1 h. We identified several eicosanoids, such as 12-HETE, 15-(S)-HETE, 8-(S)-HETE, 12-oxo-HETE, (±)13-HODE and 12-(S)-HEPE as QIs for a pre-centrifugation delay > 2 h. 12-HETE, 12-oxo-HETE, 8-(S)-HETE, and 12-(S)-HEPE, and the HI- and HG-ratios could be validated in patient samples.show moreshow less

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Metadaten
Author: Sven Heiling, Nadine Knutti, Franziska Scherr, Jörg Geiger, Juliane Weikert, Michael Rose, Roland Jahns, Uta Ceglarek, André Scherag, Michael Kiehntopf
URN:urn:nbn:de:bvb:20-opus-246261
Document Type:Journal article
Faculties:Medizinische Fakultät
Language:English
Parent Title (English):Metabolites
ISSN:2218-1989
Year of Completion:2021
Volume:11
Issue:9
Article Number:638
Source:Metabolites (2021) 11:9, 638. https://doi.org/10.3390/metabo11090638
DOI:https://doi.org/10.3390/metabo11090638
Sonstige beteiligte Institutionen:Interdisciplinary Bank of Biological Material and Data Würzburg (IBDW)
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Tag:biomarker; eicosanoids; guanosine; hypoxanthine; inosine; pre-analytical variation; quality indicators; time-to-centrifugation
Release Date:2023/05/25
Date of first Publication:2021/09/18
Licence (German):License LogoCC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International