Polyoxazolines with a vicinally double-bioactivated terminus for biomacromolecular affinity assessment

Please always quote using this URN: urn:nbn:de:bvb:20-opus-239530
  • Interactions between proteins and carbohydrates with larger biomacromolecules, e.g., lectins, are usually examined using self-assembled monolayers on target gold surfaces as a simplified model measuring setup. However, most of those measuring setups are either limited to a single substrate or do not allow for control over ligand distance and spacing. Here, we develop a synthetic strategy, consisting of a cascade of a thioesterification, native chemical ligation (NCL) and thiol-ene reaction, in order to create three-component polymer conjugatesInteractions between proteins and carbohydrates with larger biomacromolecules, e.g., lectins, are usually examined using self-assembled monolayers on target gold surfaces as a simplified model measuring setup. However, most of those measuring setups are either limited to a single substrate or do not allow for control over ligand distance and spacing. Here, we develop a synthetic strategy, consisting of a cascade of a thioesterification, native chemical ligation (NCL) and thiol-ene reaction, in order to create three-component polymer conjugates with a defined double bioactivation at the chain end. The target architecture is the vicinal attachment of two biomolecule residues to the α telechelic end point of a polymer and a thioether group at the ω chain end for fixating the conjugate to a gold sensor chip surface. As proof-of-principle studies for affinity measurements, we demonstrate the interaction between covalently bound mannose and ConA in surface acoustic wave (SAW) and surface plasmon resonance (SPR) experiments.show moreshow less

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Metadaten
Author: Florian Pinzner, Thorsten Keller, Jürgen Mut, Julian Bechold, Jürgen Seibel, Jürgen Groll
URN:urn:nbn:de:bvb:20-opus-239530
Document Type:Journal article
Faculties:Fakultät für Chemie und Pharmazie / Institut für Organische Chemie
Medizinische Fakultät / Abteilung für Funktionswerkstoffe der Medizin und der Zahnheilkunde
Language:English
Parent Title (English):Sensors
ISSN:1424-8220
Year of Completion:2021
Volume:21
Issue:9
Article Number:3153
Source:Sensors (2021) 21:9, 3153. https://doi.org/10.3390/s21093153
DOI:https://doi.org/10.3390/s21093153
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften
Tag:functionalization; lectin; polyoxazolines
Release Date:2022/07/13
Date of first Publication:2021/05/01
Licence (German):License LogoCC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International