Metabolomics in postmortem cerebrospinal fluid diagnostics: a state-of-the-art method to interpret central nervous system–related pathological processes

Please always quote using this URN: urn:nbn:de:bvb:20-opus-235724
  • In the last few years, quantitative analysis of metabolites in body fluids using LC/MS has become an established method in laboratory medicine and toxicology. By preparing metabolite profiles in biological specimens, we are able to understand pathophysiological mechanisms at the biochemical and thus the functional level. An innovative investigative method, which has not yet been used widely in the forensic context, is to use the clinical application of metabolomics. In a metabolomic analysis of 41 samples of postmortem cerebrospinal fluid (CSF)In the last few years, quantitative analysis of metabolites in body fluids using LC/MS has become an established method in laboratory medicine and toxicology. By preparing metabolite profiles in biological specimens, we are able to understand pathophysiological mechanisms at the biochemical and thus the functional level. An innovative investigative method, which has not yet been used widely in the forensic context, is to use the clinical application of metabolomics. In a metabolomic analysis of 41 samples of postmortem cerebrospinal fluid (CSF) samples divided into cohorts of four different causes of death, namely, cardiovascular fatalities, isoIated torso trauma, traumatic brain injury, and multi-organ failure, we were able to identify relevant differences in the metabolite profile between these individual groups. According to this preliminary assessment, we assume that information on biochemical processes is not gained by differences in the concentration of individual metabolites in CSF, but by a combination of differently distributed metabolites forming the perspective of a new generation of biomarkers for diagnosing (fatal) TBI and associated neuropathological changes in the CNS using CSF samples.show moreshow less

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Metadaten
Author: Simone Bohnert, Christoph Reinert, Stefanie Trella, Werner Schmitz, Benjamin Ondruschka, Michael Bohnert
URN:urn:nbn:de:bvb:20-opus-235724
Document Type:Journal article
Faculties:Medizinische Fakultät / Institut für Rechtsmedizin
Fakultät für Biologie / Theodor-Boveri-Institut für Biowissenschaften
Language:English
Parent Title (English):International Journal of Legal Medicine
ISSN:0937-9827
Year of Completion:2021
Volume:135
Pagenumber:183–191
Source:International Journal of Legal Medicine (2021) 135:183–191. https://doi.org/10.1007/s00414-020-02462-2
DOI:https://doi.org/10.1007/s00414-020-02462-2
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Tag:CSF; biomarker; cerebrospinal fluid; forensic neuropathology; forensic neurotraumatology; metabolomics
Release Date:2021/06/28
Licence (German):License LogoCC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International