DNA metabarcoding for biodiversity monitoring in a national park: Screening for invasive and pest species

Please always quote using this URN: urn:nbn:de:bvb:20-opus-217812
  • DNA metabarcoding was utilized for a large‐scale, multiyear assessment of biodiversity in Malaise trap collections from the Bavarian Forest National Park (Germany, Bavaria). Principal component analysis of read count‐based biodiversities revealed clustering in concordance with whether collection sites were located inside or outside of the National Park. Jaccard distance matrices of the presences of barcode index numbers (BINs) at collection sites in the two survey years (2016 and 2018) were significantly correlated. Overall similar patterns inDNA metabarcoding was utilized for a large‐scale, multiyear assessment of biodiversity in Malaise trap collections from the Bavarian Forest National Park (Germany, Bavaria). Principal component analysis of read count‐based biodiversities revealed clustering in concordance with whether collection sites were located inside or outside of the National Park. Jaccard distance matrices of the presences of barcode index numbers (BINs) at collection sites in the two survey years (2016 and 2018) were significantly correlated. Overall similar patterns in the presence of total arthropod BINs, as well as BINs belonging to four major arthropod orders across the study area, were observed in both survey years, and are also comparable with results of a previous study based on DNA barcoding of Sanger‐sequenced specimens. A custom reference sequence library was assembled from publicly available data to screen for pest or invasive arthropods among the specimens or from the preservative ethanol. A single 98.6% match to the invasive bark beetle Ips duplicatus was detected in an ethanol sample. This species has not previously been detected in the National Park.show moreshow less

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Metadaten
Author: Laura A. Hardulak, Jérôme Morinière, Axel Hausmann, Lars Hendrich, Stefan Schmidt, Dieter Doczkal, Jörg Müller, Paul D. N. Hebert, Gerhard Haszprunar
URN:urn:nbn:de:bvb:20-opus-217812
Document Type:Journal article
Faculties:Fakultät für Biologie / Theodor-Boveri-Institut für Biowissenschaften
Language:English
Parent Title (English):Molecular Ecology Resources
Year of Completion:2020
Volume:20
Issue:6
First Page:1542
Last Page:1557
Source:Molecular Ecology Resources 2020, 20(6):1542-1557. DOI: 10.1111/1755-0998.13212
DOI:https://doi.org/10.1111/1755-0998.13212
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
Tag:DNA barcoding; biodiversity; invasive species; metabarcoding; monitoring; pest species
Release Date:2021/07/20
Licence (German):License LogoCC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International