Localization of PD‐L1 on single cancer cells by iSERS microscopy with Au/Au core/satellite nanoparticles

Please always quote using this URN: urn:nbn:de:bvb:20-opus-212655
  • Programmed cell death‐ligand 1 (PD‐L1) is an important predictive biomarker. The detection of PD‐L1 can be crucial for patients with advanced cancer where the use of immunotherapy is considered. Here, we demonstrate the use of immuno‐SERS microscopy (iSERS) for localizing PD‐L1 on single cancer SkBr‐3 cells. A central advantage of iSERS is that the disturbing autofluorescence from cells and tissues can be efficiently minimized by red to near‐infrared laser excitation. In this study we employed Au/Au core/satellite nanoparticles as SERS nanotagsProgrammed cell death‐ligand 1 (PD‐L1) is an important predictive biomarker. The detection of PD‐L1 can be crucial for patients with advanced cancer where the use of immunotherapy is considered. Here, we demonstrate the use of immuno‐SERS microscopy (iSERS) for localizing PD‐L1 on single cancer SkBr‐3 cells. A central advantage of iSERS is that the disturbing autofluorescence from cells and tissues can be efficiently minimized by red to near‐infrared laser excitation. In this study we employed Au/Au core/satellite nanoparticles as SERS nanotags because of their remarkable signal brightness and colloidal stability upon red laser excitation. False‐color iSERS images of the positive and negative controls clearly reveal the specific localization of PD‐L1 with SERS nanotag‐labeled antibodies.show moreshow less

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Metadaten
Author: Elzbieta Stepula, Matthias König, Xin‐Ping Wang, Janina Levermann, Tobias Schimming, Sabine Kasimir‐Bauer, Bastian Schilling, Sebastian Schlücker
URN:urn:nbn:de:bvb:20-opus-212655
Document Type:Journal article
Faculties:Medizinische Fakultät / Klinik und Poliklinik für Dermatologie, Venerologie und Allergologie
Language:English
Parent Title (English):Journal of Biophotonics
Year of Completion:2020
Volume:13
Issue:3
Article Number:e201960034
Source:Journal of Biophotonics 2020, 13(3):e201960034. DOI: 10.1002/jbio.201960034
DOI:https://doi.org/10.1002/jbio.201960034
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Tag:PD‐L1; Raman; SERS; gold nanoparticles
Release Date:2021/04/15
Licence (German):License LogoCC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International