Chromosome Banding in Amphibia. XXXIII. Demonstration of 5-Methylcytosine-Rich Heterochromatin in Anura

Please always quote using this URN: urn:nbn:de:bvb:20-opus-199022
  • An experimental approach using monoclonal anti-5-methylcytosine (5-MeC) antibodies and indirect immunofluorescence was elaborated for detecting 5-MeC-rich chromosome regions in anuran chromosomes. This technique was applied to mitotic metaphases of 6 neotropical frog species belonging to 6 genera and 4 families. The hypermethylation patterns were compared with a variety of banding patterns obtained by conventional banding techniques. The hypermethylated DNA sequences are species-specific and located exclusively in constitutive heterochromatin.An experimental approach using monoclonal anti-5-methylcytosine (5-MeC) antibodies and indirect immunofluorescence was elaborated for detecting 5-MeC-rich chromosome regions in anuran chromosomes. This technique was applied to mitotic metaphases of 6 neotropical frog species belonging to 6 genera and 4 families. The hypermethylation patterns were compared with a variety of banding patterns obtained by conventional banding techniques. The hypermethylated DNA sequences are species-specific and located exclusively in constitutive heterochromatin. They are found in centromeric, pericentromeric, telomeric, and interstitial positions of the chromosomes and adjacent to nucleolus organizer regions. 5-MeC-rich DNA sequences can be embedded both in AT- and GC-rich repetitive DNA. The experimental parameters that have major influence on the reproducibility and quality of the anti-5-MeC antibody labeling are discussed.show moreshow less

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Metadaten
Author: Michael Schmid, Claus Steinlein
URN:urn:nbn:de:bvb:20-opus-199022
Document Type:Journal article
Faculties:Medizinische Fakultät / Institut für Humangenetik
Language:English
Parent Title (English):Cytogenetic and Genome Research
ISSN:1424-8581
ISSN:1424-859X
Year of Completion:2016
Volume:148
Issue:1
Pagenumber:35-43
Source:Cytogenetic and Genome Research 2016;148(1):35–43. DOI: 10.1159/000446141
DOI:https://doi.org/10.1159/000446141
Pubmed Id:https://pubmed.ncbi.nlm.nih.gov/27160320
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Tag:5-Methylcytosine; Anura; heterochromatin; hypermethylated DNA; immunofluorescence
Release Date:2021/08/20
Date of first Publication:2016/04/30
Note:
This publication is with permission of the rights owner freely accessible due to an Alliance licence and a national licence (funded by the DFG, German Research Foundation) respectively.
Licence (German):License LogoDeutsches Urheberrecht