Anisotropic cryostructured collagen scaffolds for efficient delivery of RhBMP−2 and enhanced bone regeneration

Please always quote using this URN: urn:nbn:de:bvb:20-opus-195966
  • In the treatment of bone non-unions, an alternative to bone autografts is the use of bone morphogenetic proteins (BMPs), e.g., BMP–2, BMP–7, with powerful osteoinductive and osteogenic properties. In clinical settings, these osteogenic factors are applied using absorbable collagen sponges for local controlled delivery. Major side effects of this strategy are derived from the supraphysiological doses of BMPs needed, which may induce ectopic bone formation, chronic inflammation, and excessive bone resorption. In order to increase the efficiencyIn the treatment of bone non-unions, an alternative to bone autografts is the use of bone morphogenetic proteins (BMPs), e.g., BMP–2, BMP–7, with powerful osteoinductive and osteogenic properties. In clinical settings, these osteogenic factors are applied using absorbable collagen sponges for local controlled delivery. Major side effects of this strategy are derived from the supraphysiological doses of BMPs needed, which may induce ectopic bone formation, chronic inflammation, and excessive bone resorption. In order to increase the efficiency of the delivered BMPs, we designed cryostructured collagen scaffolds functionalized with hydroxyapatite, mimicking the structure of cortical bone (aligned porosity, anisotropic) or trabecular bone (random distributed porosity, isotropic). We hypothesize that an anisotropic structure would enhance the osteoconductive properties of the scaffolds by increasing the regenerative performance of the provided rhBMP–2. In vitro, both scaffolds presented similar mechanical properties, rhBMP–2 retention and delivery capacity, as well as scaffold degradation time. In vivo, anisotropic scaffolds demonstrated better bone regeneration capabilities in a rat femoral critical-size defect model by increasing the defect bridging. In conclusion, anisotropic cryostructured collagen scaffolds improve bone regeneration by increasing the efficiency of rhBMP–2 mediated bone healing.show moreshow less

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Metadaten
Author: Kai Stuckensen, José M. Lamo-Espinosa, Emma Muiños-López, Purificación Ripalda-Cemboráin, Tania López-Martínez, Elena Iglesias, Gloria Abizanda, Ion Andreu, María Flandes-Iparraguirre, Juan Pons-Villanueva, Reyes Elizalde, Joachim Nickel, Andrea Ewald, Uwe Gbureck, Felipe Prósper, Jürgen Groll, Froilán Granero-Moltó
URN:urn:nbn:de:bvb:20-opus-195966
Document Type:Journal article
Faculties:Medizinische Fakultät / Lehrstuhl für Tissue Engineering und Regenerative Medizin
Medizinische Fakultät / Abteilung für Funktionswerkstoffe der Medizin und der Zahnheilkunde
Language:English
Parent Title (English):Materials
ISSN:1996-1944
Year of Completion:2019
Volume:12
Issue:19
Article Number:3105
Source:Materials (2019) 12:19, 3105. https://doi.org/10.3390/ma12193105
DOI:https://doi.org/10.3390/ma12193105
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Tag:bone critical size defect; collagen sponge; cryostructured scaffolds; rhBMP–2
Release Date:2022/05/23
Date of first Publication:2019/09/24
EU-Project number / Contract (GA) number:309962
OpenAIRE:OpenAIRE
Licence (German):License LogoCC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International