Distinct increased outliers among 136 rectal cancer patients assessed by \(\gamma\)H2AX

Please always quote using this URN: urn:nbn:de:bvb:20-opus-144085
  • Background: In recent years attention has focused on \(\gamma\)H2AX as a very sensitive double strand break indicator. It has been suggested that \(\gamma\)H2AX might be able to predict individual radiosensitivity. Our aim was to study the induction and repair of DNA double strand breaks labelled by \(\gamma\)H2AX in a large cohort. Methods: In a prospective study lymphocytes of 136 rectal cancer (RC) patients and 59 healthy individuals were ex vivo irradiated (IR) and initial DNA damage was compared to remaining DNA damage after 2 GyBackground: In recent years attention has focused on \(\gamma\)H2AX as a very sensitive double strand break indicator. It has been suggested that \(\gamma\)H2AX might be able to predict individual radiosensitivity. Our aim was to study the induction and repair of DNA double strand breaks labelled by \(\gamma\)H2AX in a large cohort. Methods: In a prospective study lymphocytes of 136 rectal cancer (RC) patients and 59 healthy individuals were ex vivo irradiated (IR) and initial DNA damage was compared to remaining DNA damage after 2 Gy and 24 hours repair time and preexisting DNA damage in unirradiated lymphocytes. Lymphocytes were immunostained with anti-\(\gamma\)H2AX antibodies and microscopic images with an extended depth of field were acquired. \(\gamma\)H2AX foci counting was performed using a semi-automatic image analysis software. Results: Distinct increased values of preexisting and remaining \(\gamma\)H2AX foci in the group of RC patients were found compared to the healthy individuals. Additionally there are clear differences within the groups and there are outliers in about 12% of the RC patients after ex vivo IR. Conclusions: The \(\gamma\)H2AX assay has the capability to identify a group of outliers which are most probably patients with increased radiosensitivity having the highest risk of suffering radiotherapy-related late sequelae.show moreshow less

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Metadaten
Author: Jana Kroeber, Barbara Wenger, Manuela Schwegler, Christoph Daniel, Manfred Schmidt, Cholpon S Djuzenova, Bülent Polat, Michael Flentje, Rainer Fietkau, Luitpold V. Distel
URN:urn:nbn:de:bvb:20-opus-144085
Document Type:Journal article
Faculties:Medizinische Fakultät / Klinik und Poliklinik für Strahlentherapie
Language:English
Parent Title (English):Radiation Oncology
Year of Completion:2015
Volume:10
Issue:36
Source:Radiation Oncology (2015) 10:36. DOI: 10.1186/s13014-015-0344-5
DOI:https://doi.org/10.1186/s13014-015-0344-5
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Tag:DNA damage; DNA double strand breaks; \(\gamma\)h2ax; blood lymphocytes; foci; histone H2AX; in vivo; individual radiosensitivity; neck cancer; oral mucositis; phosphorylation; radiosensitivity; radiotherapy; rectal cancer; repair
Release Date:2018/06/04
Licence (German):License LogoCC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International