Identification of Eps15 as Antigen Recognized by the Monoclonal Antibodies aa2 and ab52 of the Wuerzburg Hybridoma Library against Drosophila Brain

Please always quote using this URN: urn:nbn:de:bvb:20-opus-137957
  • The Wuerzburg Hybridoma Library against the Drosophila brain represents a collection of around 200 monoclonal antibodies that bind to specific structures in the Drosophila brain. Here we describe the immunohistochemical staining patterns, the Western blot signals of one- and two-dimensional electrophoretic separation, and the mass spectrometric characterization of the target protein candidates recognized by the monoclonal antibodies aa2 and ab52 from the library. Analysis of a mutant of a candidate gene identified the Drosophila homolog of theThe Wuerzburg Hybridoma Library against the Drosophila brain represents a collection of around 200 monoclonal antibodies that bind to specific structures in the Drosophila brain. Here we describe the immunohistochemical staining patterns, the Western blot signals of one- and two-dimensional electrophoretic separation, and the mass spectrometric characterization of the target protein candidates recognized by the monoclonal antibodies aa2 and ab52 from the library. Analysis of a mutant of a candidate gene identified the Drosophila homolog of the Epidermal growth factor receptor Pathway Substrate clone 15 (Eps15) as the antigen for these two antibodies.show moreshow less

Download full text files

Export metadata

Additional Services

Share in Twitter Search Google Scholar Statistics
Metadaten
Author: Halder Partho, Yi-chun Chen, Janine Brauckhoff, Alois Hofbauer, Marie-Christine Dabauvalle, Urs Lewandrowski, Christiane Winkler, Albert Sickmann, Erich Buchner
URN:urn:nbn:de:bvb:20-opus-137957
Document Type:Journal article
Faculties:Medizinische Fakultät / Institut für Klinische Neurobiologie
Fakultät für Biologie / Theodor-Boveri-Institut für Biowissenschaften
Fakultät für Biologie / Rudolf-Virchow-Zentrum
Language:English
Parent Title (English):PLoS One
Year of Completion:2011
Volume:6
Issue:12
Pagenumber:e29352
Source:PLoS ONE 6(12): e29352. doi:10.1371/journal.pone.0029352
DOI:https://doi.org/10.1371/journal.pone.0029352
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 59 Tiere (Zoologie) / 595 Arthropoden (Gliederfüßer)
Tag:antigen processing and recognition; drosophila melanogaster; gel electrophoresis; hybridomas; immunohistochemistry techniques; immunoprecipitation; neuropil; silver staining
Release Date:2016/08/29
Collections:Open-Access-Publikationsfonds / Förderzeitraum 2011
Licence (German):License LogoCC BY: Creative-Commons-Lizenz: Namensnennung