cAMP signaling microdomains and their observation by optical methods

Please always quote using this URN: urn:nbn:de:bvb:20-opus-118252
  • The second messenger cyclic AMP (cAMP) is a major intracellular mediator of many hormones and neurotransmitters and regulates a myriad of cell functions, including synaptic plasticity in neurons. Whereas cAMP can freely diffuse in the cytosol, a growing body of evidence suggests the formation of cAMP gradients and microdomains near the sites of cAMP production, where cAMP signals remain apparently confined. The mechanisms responsible for the formation of such microdomains are subject of intensive investigation. The development of opticalThe second messenger cyclic AMP (cAMP) is a major intracellular mediator of many hormones and neurotransmitters and regulates a myriad of cell functions, including synaptic plasticity in neurons. Whereas cAMP can freely diffuse in the cytosol, a growing body of evidence suggests the formation of cAMP gradients and microdomains near the sites of cAMP production, where cAMP signals remain apparently confined. The mechanisms responsible for the formation of such microdomains are subject of intensive investigation. The development of optical methods based on fluorescence resonance energy transfer (FRET), which allow a direct observation of cAMP signaling with high temporal and spatial resolution, is playing a fundamental role in elucidating the nature of such microdomains. Here, we will review the optical methods used for monitoring cAMP and protein kinase A (PKA) signaling in living cells, providing some examples of their application in neurons, and will discuss the major hypotheses on the formation of cAMP/PKA microdomains.show moreshow less

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Metadaten
Author: Davide Calebiro, Isabella Maiellaro
URN:urn:nbn:de:bvb:20-opus-118252
Document Type:Journal article
Faculties:Medizinische Fakultät / Institut für Pharmakologie und Toxikologie
Fakultät für Biologie / Rudolf-Virchow-Zentrum
Language:English
Parent Title (English):Frontiers in Cellular Neuroscience
ISSN:1662-5102
Year of Completion:2014
Volume:8
Pagenumber:350
Source:Frontiers in Cellular Neuroscience 8:350. doi: 10.3389/fncel.2014.00350
DOI:https://doi.org/10.3389/fncel.2014.00350
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 571 Physiologie und verwandte Themen
6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 611 Menschliche Anatomie, Zytologie, Histologie
Tag:G protein-coupled receptor; cyclic AMP; fluorescence resonance energy transfer; neurons; signaling microdomain
Release Date:2015/10/02
Licence (German):License LogoCC BY: Creative-Commons-Lizenz: Namensnennung