Guidance of Mesenchymal Stem Cells on Fibronectin Structured Hydrogel Films

Please always quote using this URN: urn:nbn:de:bvb:20-opus-114897
  • Designing of implant surfaces using a suitable ligand for cell adhesion to stimulate specific biological responses of stem cells will boost the application of regenerative implants. For example, materials that facilitate rapid and guided migration of stem cells would promote tissue regeneration. When seeded on fibronectin (FN) that was homogeneously immmobilized to NCO-sP(EO-stat-PO), which otherwise prevents protein binding and cell adhesion, human mesenchymal stem cells (MSC) revealed a faster migration, increased spreading and a more rapidDesigning of implant surfaces using a suitable ligand for cell adhesion to stimulate specific biological responses of stem cells will boost the application of regenerative implants. For example, materials that facilitate rapid and guided migration of stem cells would promote tissue regeneration. When seeded on fibronectin (FN) that was homogeneously immmobilized to NCO-sP(EO-stat-PO), which otherwise prevents protein binding and cell adhesion, human mesenchymal stem cells (MSC) revealed a faster migration, increased spreading and a more rapid organization of different cellular components for cell adhesion on fibronectin than on a glass surface. To further explore, how a structural organization of FN controls the behavior of MSC, adhesive lines of FN with varying width between 10 mu m and 80 mu m and spacings between 5 mu m and 20 mu m that did not allow cell adhesion were generated. In dependance on both line width and gaps, cells formed adjacent cell contacts, were individually organized in lines, or bridged the lines. With decreasing sizes of FN lines, speed and directionality of cell migration increased, which correlated with organization of the actin cytoskeleton, size and shape of the nuclei as well as of focal adhesions. Together, defined FN lines and gaps enabled a fine tuning of the structural organization of cellular components and migration. Microstructured adhesive substrates can mimic the extracellular matrix in vivo and stimulate cellular mechanisms which play a role in tissue regeneration.show moreshow less

Download full text files

Export metadata

Additional Services

Share in Twitter Search Google Scholar Statistics
Metadaten
Author: Annika Kasten, Tamara Naser, Kristina Brüllhoff, Jörg Fiedler, Petra Müller, Martin Möller, Joachim Rychly, Jürgen Groll, Rolf E. Brenner
URN:urn:nbn:de:bvb:20-opus-114897
Document Type:Journal article
Faculties:Medizinische Fakultät / Abteilung für Funktionswerkstoffe der Medizin und der Zahnheilkunde
Language:English
Parent Title (English):PLOS ONE
Year of Completion:2014
Volume:9
Issue:10
Pagenumber:e109411
Source:PLoS ONE 9(10): e109411. doi:10.1371/journal.pone.0109411
DOI:https://doi.org/10.1371/journal.pone.0109411
Pubmed Id:https://pubmed.ncbi.nlm.nih.gov/25329487
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Tag:adhesion dynamics; coatings; focal adhesions; force; migration; networks; stress; tension; tissue morphogenesis; traction
Release Date:2015/07/10
Licence (German):License LogoCC BY: Creative-Commons-Lizenz: Namensnennung