Ablasser et al., 2009 A. Ablasser, H. Poeck, D. Anz, M. Berger, M. Schlee, S. Kim, C. Bourquin, N. Goutagny, Z. Jiang, K.A. Fitzgerald, et al.Selection of molecular structure and delivery of RNA oligonucleotides to activate TLR7 versus TLR8 and to induce high amounts of IL-12p70 in primary human monocytes J. Immunol., 182 (2009), pp. 6824-6833 Ahn et al., 2012 J. Ahn, D. Gutman, S. Saijo, G.N. BarberSTING manifests self DNA-dependent inflammatory disease Proc. Natl. Acad. Sci. USA, 109 (2012), pp. 19386-19391 Barbalat et al., 2011 R. Barbalat, S.E. Ewald, M.L. Mouchess, G.M. BartonNucleic acid recognition by the innate immune system Annu. Rev. Immunol., 29 (2011), pp. 185-214 Bergstrøm et al., 2015 B. Bergstrøm, M.H. Aune, J.A. Awuh, J.F. Kojen, K.J. Blix, L. Ryan, T.H. Flo, T.E. Mollnes, T. Espevik, J. StenvikTLR8 Senses Staphylococcus aureus RNA in Human Primary Monocytes and Macrophages and Induces IFN-β Production via a TAK1-IKKβ-IRF5 Signaling Pathway J. Immunol., 195 (2015), pp. 1100-1111 Chan et al., 2015 M.P. Chan, M. Onji, R. Fukui, K. Kawane, T. Shibata, S. Saitoh, U. Ohto, T. Shimizu, G.N. Barber, K. MiyakeDNase II-dependent DNA digestion is required for DNA sensing by TLR9 Nat. Commun., 6 (2015), p. 5853 Crow and Manel, 2015 Y.J. Crow, N. ManelAicardi-Goutières syndrome and the type I interferonopathies Nat. Rev. Immunol., 15 (2015), pp. 429-440 Diebold et al., 2004 S.S. Diebold, T. Kaisho, H. Hemmi, S. Akira, C. Reis e SousaInnate antiviral responses by means of TLR7-mediated recognition of single-stranded RNA Science, 303 (2004), pp. 1529-1531 Dobin et al., 2013 A. Dobin, C.A. Davis, F. Schlesinger, J. Drenkow, C. Zaleski, S. Jha, P. Batut, M. Chaisson, T.R. GingerasSTAR: ultrafast universal RNA-seq aligner Bioinformatics, 29 (2013), pp. 15-21 Forsbach et al., 2008 A. Forsbach, J.G. Nemorin, C. Montino, C. Müller, U. Samulowitz, A.P. Vicari, M. Jurk, G.K. Mutwiri, A.M. Krieg, G.B. Lipford, J. VollmerIdentification of RNA sequence motifs stimulating sequence-specific TLR8-dependent immune responses J. Immunol., 180 (2008), pp. 3729-3738 Gaidt et al., 2016 M.M. Gaidt, T.S. Ebert, D. Chauhan, T. Schmidt, J.L. Schmid-Burgk, F. Rapino, A.A. Robertson, M.A. Cooper, T. Graf, V. HornungHuman Monocytes Engage an Alternative Inflammasome Pathway Immunity, 44 (2016), pp. 833-846 Gaidt et al., 2017 M.M. Gaidt, T.S. Ebert, D. Chauhan, K. Ramshorn, F. Pinci, S. Zuber, F. O’Duill, J.L. Schmid-Burgk, F. Hoss, R. Buhmann, et al.The DNA Inflammasome in Human Myeloid Cells Is Initiated by a STING-Cell Death Program Upstream of NLRP3 Cell, 171 (2017), pp. 1110-1124.e18 Gao et al., 2015 D. Gao, T. Li, X.D. Li, X. Chen, Q.Z. Li, M. Wight-Carter, Z.J. ChenActivation of cyclic GMP-AMP synthase by self-DNA causes autoimmune diseases Proc. Natl. Acad. Sci. USA, 112 (2015), pp. E5699-E5705 Globisch et al., 2011 D. Globisch, D. Pearson, A. Hienzsch, T. Brückl, M. Wagner, I. Thoma, P. Thumbs, V. Reiter, A.C. Kneuttinger, M. Müller, et al.Systems-based analysis of modified tRNA bases Angew. Chem. Int. Ed. Engl., 50 (2011), pp. 9739-9742 Haud et al., 2011 N. Haud, F. Kara, S. Diekmann, M. Henneke, J.R. Willer, M.S. Hillwig, R.G. Gregg, G.C. Macintosh, J. Gärtner, A. Alia, A.F. Hurlstonernaset2 mutant zebrafish model familial cystic leukoencephalopathy and reveal a role for RNase T2 in degrading ribosomal RNA Proc. Natl. Acad. Sci. USA, 108 (2011), pp. 1099-1103 Heil et al., 2004 F. Heil, H. Hemmi, H. Hochrein, F. Ampenberger, C. Kirschning, S. Akira, G. Lipford, H. Wagner, S. BauerSpecies-specific recognition of single-stranded RNA via toll-like receptor 7 and 8 Science, 303 (2004), pp. 1526-1529 Henneke et al., 2009 M. Henneke, S. Diekmann, A. Ohlenbusch, J. Kaiser, V. Engelbrecht, A. Kohlschütter, R. Krätzner, M. Madruga-Garrido, M. Mayer, L. Opitz, et al.RNASET2-deficient cystic leukoencephalopathy resembles congenital cytomegalovirus brain infection Nat. Genet., 41 (2009), pp. 773-775 Krüger et al., 2015 A. Krüger, M. Oldenburg, C. Chebrolu, D. Beisser, J. Kolter, A.M. Sigmund, J. Steinmann, S. Schäfer, H. Hochrein, S. Rahmann, et al.Human TLR8 senses UR/URR motifs in bacterial and mitochondrial RNA EMBO Rep., 16 (2015), pp. 1656-1663 Li and Dewey, 2011 B. Li, C.N. DeweyRSEM: accurate transcript quantification from RNA-Seq data with or without a reference genome BMC Bioinformatics, 12 (2011), p. 323 Li et al., 2013 Z. Li, I.P. Michael, D. Zhou, A. Nagy, J.M. RiniSimple piggyBac transposon-based mammalian cell expression system for inducible protein production Proc. Natl. Acad Sci. U S A, 110 (2013), pp. 5004-5009 MacIntosh, 2011 G.C. MacIntoshRNase T2 Family: Enzymatic Properties, Functional Diversity, and Evolution of Ancient Ribonucleases A. Nicholson (Ed.), Ribonucleases. Nucleic Acids and Molecular Biology, Springer Berlin Heidelberg (2011), pp. 89-114 Numata et al., 2003 T. Numata, A. Suzuki, Y. Kakuta, K. Kimura, M. Yao, I. Tanaka, Y. Yoshida, T. Ueda, M. KimuraCrystal structures of the ribonuclease MC1 mutants N71T and N71S in complex with 5′-GMP: structural basis for alterations in substrate specificity Biochemistry, 42 (2003), pp. 5270-5278 Oldenburg et al., 2012 M. Oldenburg, A. Krüger, R. Ferstl, A. Kaufmann, G. Nees, A. Sigmund, B. Bathke, H. Lauterbach, M. Suter, S. Dreher, et al.TLR13 recognizes bacterial 23S rRNA devoid of erythromycin resistance-forming modification Science, 337 (2012), pp. 1111-1115 Rapino et al., 2013 F. Rapino, E.F. Robles, J.A. Richter-Larrea, E.M. Kallin, J.A. Martinez-Climent, T. GrafC/EBPα induces highly efficient macrophage transdifferentiation of B lymphoma and leukemia cell lines and impairs their tumorigenicity Cell Rep., 3 (2013), pp. 1153-1163 Rice et al., 2017 G.I. Rice, I. Melki, M.L. Frémond, T.A. Briggs, M.P. Rodero, N. Kitabayashi, A. Oojageer, B. Bader-Meunier, A. Belot, C. Bodemer, et al.Assessment of Type I Interferon Signaling in Pediatric Inflammatory Disease J. Clin. Immunol., 37 (2017), pp. 123-132 Roers et al., 2016 A. Roers, B. Hiller, V. HornungRecognition of Endogenous Nucleic Acids by the Innate Immune System Immunity, 44 (2016), pp. 739-754 Schmid-Burgk et al., 2014 J.L. Schmid-Burgk, T. Schmidt, M.M. Gaidt, K. Pelka, E. Latz, T.S. Ebert, V. HornungOutKnocker: a web tool for rapid and simple genotyping of designer nuclease edited cell lines Genome Res., 24 (2014), pp. 1719-1723 Schmidt et al., 2015 T. Schmidt, J.L. Schmid-Burgk, V. HornungSynthesis of an arrayed sgRNA library targeting the human genome Scientific reports, 5 (2015), p. 14987 Song et al., 2015 W. Song, J. Wang, Z. Han, Y. Zhang, H. Zhang, W. Wang, J. Chang, B. Xia, S. Fan, D. Zhang, et al.Structural basis for specific recognition of single-stranded RNA by Toll-like receptor 13 Nat. Struct. Mol. Biol., 22 (2015), pp. 782-787 Suzuki et al., 2000 A. Suzuki, M. Yao, I. Tanaka, T. Numata, S. Kikukawa, N. Yamasaki, M. KimuraCrystal structures of the ribonuclease MC1 from bitter gourd seeds, complexed with 2′-UMP or 3′-UMP, reveal structural basis for uridine specificity Biochem. Biophys. Res. Commun., 275 (2000), pp. 572-576 Tanji et al., 2015 H. Tanji, U. Ohto, T. Shibata, M. Taoka, Y. Yamauchi, T. Isobe, K. Miyake, T. ShimizuToll-like receptor 8 senses degradation products of single-stranded RNA Nat. Struct. Mol. Biol., 22 (2015), pp. 109-115 Thorn et al., 2012 A. Thorn, R. Steinfeld, M. Ziegenbein, M. Grapp, H.H. Hsiao, H. Urlaub, G.M. Sheldrick, J. Gärtner, R. KrätznerStructure and activity of the only human RNase T2 Nucleic Acids Res., 40 (2012), pp. 8733-8742 Wu and Chen, 2014 J. Wu, Z.J. ChenInnate immune sensing and signaling of cytosolic nucleic acids Annu. Rev. Immunol., 32 (2014), pp. 461-488 Zhang et al., 2016 Z. Zhang, U. Ohto, T. Shibata, E. Krayukhina, M. Taoka, Y. Yamauchi, H. Tanji, T. Isobe, S. Uchiyama, K. Miyake, T. ShimizuStructural Analysis Reveals that Toll-like Receptor 7 Is a Dual Receptor for Guanosine and Single-Stranded RNA Immunity, 45 (2016), pp. 737-748 Zhang et al., 2018 Z. Zhang, U. Ohto, T. Shibata, M. Taoka, Y. Yamauchi, R. Sato, N.M. Shukla, S.A. David, T. Isobe, K. Miyake, T. ShimizuStructural Analyses of Toll-like Receptor 7 Reveal Detailed RNA Sequence Specificity and Recognition Mechanism of Agonistic Ligands Cell Rep., 25 (2018), pp. 3371-3381.e5