- AutorIn
- Elisabeth Christiane Seidel
- Claudia Birkemeyer
- Rainer Baran-Schmidt
- Jürgen Meixensberger
- Henry Oppermann
- Frank Gaunitz
- Titel
- Viability of Glioblastoma Cells and Fibroblasts in the Presence of Imidazole-Containing Compounds
- Zitierfähige Url:
- https://nbn-resolving.org/urn:nbn:de:bsz:15-qucosa2-891348
- Quellenangabe
- International Journal of Molecular Sciences
Erscheinungsjahr: 2022
Jahrgang: 23
Artikelnummer: 5834 - Erstveröffentlichung
- 2022
- Abstract (EN)
- The naturally occurring dipeptide carnosine (-alanyl-L-histidine) specifically attenuates tumor growth. Here, we ask whether other small imidazole-containing compounds also affect the viability of tumor cells without affecting non-malignant cells and whether the formation of histamine is involved. Patient-derived fibroblasts and glioblastoma cells were treated with carnosine, L-alanyl-L-histidine (LA-LH), -alanyl-L-alanine, L-histidine, histamine, imidazole, -alanine, and L-alanine. Cell viability was assessed by cell-based assays and microscopy. The intracellular release of L-histidine and formation of histamine was investigated by high-performance liquid chromatography coupled to mass spectrometry. Carnosine and LA-LH inhibited tumor cell growth with minor effects on fibroblasts, and L-histidine, histamine, and imidazole affected viability in both cell types. Compounds without the imidazole moiety did not diminish viability. In the presence of LA-LH but not in the presence of carnosine, a significant rise in intracellular amounts of histidine was detected in all cells. The formation of histamine was not detectable in the presence of carnosine, LA-LH, or histidine. In conclusion, the imidazole moiety of carnosine contributes to its anti-neoplastic effect, which is also seen in the presence of histidine and LA-LH. Despite the fact that histamine has a strong effect on cell viability, the formation of histamine is not responsible for the effects on the cell viability of carnosine, LA-LH, and histidine.
- Andere Ausgabe
- Link zur Erstveröffentlichung
Link: https://doi.org/10.3390/ijms23105834 - Freie Schlagwörter (EN)
- carnosine; glioblastoma; fibroblasts; imidazole-containing compounds; cell viability; high-performance liquid chromatography coupled to mass spectrometry
- Klassifikation (DDC)
- 610
- Verlag
- MDPI, Basel
- Version / Begutachtungsstatus
- publizierte Version / Verlagsversion
- URN Qucosa
- urn:nbn:de:bsz:15-qucosa2-891348
- Veröffentlichungsdatum Qucosa
- 18.01.2024
- Dokumenttyp
- Artikel
- Sprache des Dokumentes
- Englisch
- Lizenz / Rechtehinweis
- CC BY 4.0