- AutorIn
- Sethuraman Subramanian Technische Universität Dresden, Center for Molecular and Cellular Bioenigeerig (CMCB), Center for Regenerative Therapies Dresden (CRTD)
- Clara Jana-Lui BuschTechnische Universität Dresden, Center for Molecular and Cellular Bioenigeerig (CMCB), Center for Regenerative Therapies Dresden (CRTD)
- Kaaweh Molawi
- Laufey Geirsdottir
- Julien Maurizio
- Stephanie Vargas Aguilar
- Hassiba Belahbib
- Gregor Gimenez
- Ridzky Anis Advent Yuda
- Michaela Burkon
- Jérémy Favret
- Sara Gholamhosseinian Najjar
- Berengère de Laval
- Prashanth Kumar Kandalla
- Sandrine Sarrazin Zentrum für Regenerative Sarrazin
- Lena Alexopoulou
- Michael H. Siewake
- Titel
- Long-term culture-expanded alveolar macrophages restore their full epigenetic identity after transfer in vivo
- Zitierfähige Url:
- https://nbn-resolving.org/urn:nbn:de:bsz:14-qucosa2-783996
- Quellenangabe
- Nature Immunology
Erscheinungsjahr: 2022
Jahrgang: 23
Seiten: 458-468
ISSN: 1529-2908
E-ISSN: 1529-2916 - Erstveröffentlichung
- 2022
- Abstract (EN)
- Alveolar macrophages (AMs) are lung tissue-resident macrophages that can be expanded in culture, but it is unknown to what extent culture affects their in vivo identity. Here we show that mouse long-term ex vivo expanded AMs (exAMs) maintained a core AM gene expression program, but showed culture adaptations related to adhesion, metabolism and proliferation. Upon transplantation into the lung, exAMs reacquired full transcriptional and epigenetic AM identity, even after several months in culture and could self-maintain long-term in the alveolar niche. Changes in open chromatin regions observed in culture were fully reversible in transplanted exAMs and resulted in a gene expression profile indistinguishable from resident AMs. Our results indicate that long-term proliferation of AMs in culture did not compromise cellular identity in vivo. The robustness of exAM identity provides new opportunities for mechanistic analysis and highlights the therapeutic potential of exAMs.
- Andere Ausgabe
- Link zum Artikel, der zuerst in der Zeitschrift 'nature immunology' bei Springer Nature erschienen ist:
DOI: https://doi.org/10.1038/s41590-022-01146-w - Freie Schlagwörter (DE)
- Alveolarmakrophagen (AMs), Maus-Langzeit-Ex-vivo-erweiterte AMs (exAMs), transkriptionelle und epigenetische AM-Identität, Zellkultur
- Freie Schlagwörter (EN)
- Alveolar macrophages (AMs), mouse long-term ex vivo expanded AMs (exAMs), transcriptional and epigenetic AM identity, cell culture
- Klassifikation (DDC)
- 610
- Verlag
- Springer Nature, New York, NY
- Förder- / Projektangaben
- European Commission (EC)
H2020 | ERC | ERC-ADG Macrophage aging and rejuvenation
(MacAGE)
ID: 695093 - Version / Begutachtungsstatus
- angenommene Version / Postprint / Autorenversion
- URN Qucosa
- urn:nbn:de:bsz:14-qucosa2-783996
- Veröffentlichungsdatum Qucosa
- 26.08.2022
- Dokumenttyp
- Artikel
- Sprache des Dokumentes
- Englisch
- Deutsch
- Lizenz / Rechtehinweis