- AutorIn
- Martin Schmidt Technische Universität Dresden, Department of Plant Cell and Molecular Biology, Dresden, Germany
- Sarah HenseTechnische Universität Dresden, Department of Plant Cell and Molecular Biology, Dresden, Germany
- André E. Minoche
- Juliane C. Dohm
- Heinz Himmelbauer
- Thomas Schmidt
- Falk Zakrzewski
- Titel
- Cytosine Methylation of an Ancient Satellite Family in the Wild Beet Beta procumbens
- Zitierfähige Url:
- https://nbn-resolving.org/urn:nbn:de:bsz:14-qucosa2-705761
- Quellenangabe
- Cytogenetic and genome research Erscheinungsort: Basel
Verlag: Karger
Erscheinungsjahr: 2014
Jahrgang: 143
Heft: 1-3
Seiten: 157-167
ISSN: 1424-8581
E-ISSN: 1424-859X - Erstveröffentlichung
- 2014
- Abstract (EN)
- DNA methylation is an essential epigenetic feature for the regulation and maintenance of heterochromatin. Satellite DNA is a repetitive sequence component that often occurs in large arrays in heterochromatin of subtelomeric, intercalary and centromeric regions. Knowledge about the methylation status of satellite DNA is important for understanding the role of repetitive DNA in heterochromatization. In this study, we investigated the cytosine methylation of the ancient satellite family pEV in the wild beet Beta procumbens. The pEV satellite is widespread in species-specific pEV subfamilies in the genus Beta and most likely originated before the radiation of the Betoideae and Chenopodioideae. In B. procumbens , the pEV subfamily occurs abundantly and spans intercalary and centromeric regions. To uncover its cytosine methylation, we performed chromosome-wide immunostaining and bisulfite sequencing of pEV satellite repeats. We found that CG and CHG sites are highly methylated while CHH sites show only low levels of methylation. As a consequence of the low frequency of CG and CHG sites and the preferential occurrence of most cytosines in the CHH motif in pEV monomers, this satellite family displays only low levels of total cytosine methylation.
- Andere Ausgabe
- Link zum Artikel der zuerst in der Zeitschrift 'Cytogenetic and genome research' erschienen ist
DOI: 10.1159/000363485 - Freie Schlagwörter (DE)
- Beta-Procumben, Bisulfit-Sequenzierung, DNA-Methylierung, Immunfärbung, Satelliten-DNA, Zuckerrübe
- Freie Schlagwörter (EN)
- Beta procumbens, Bisulfite sequencing, DNA methylation, Immunostaining, Satellite DNA, Sugar beet
- Klassifikation (DDC)
- 610
- 570
- Verlag
- Karger, Basel
- Förder- / Projektangaben
- Bundesministerium für Bildung und Forschung
Annotation des Genoms der Zuckerrübe unter Berücksichtigung von Genfunktionen und struktureller Variabilität für die Nutzung von Genomdaten in der Pflanzenbiotechnologie (ANNOBEET)
ID: 0315962 - Version / Begutachtungsstatus
- publizierte Version / Verlagsversion
- URN Qucosa
- urn:nbn:de:bsz:14-qucosa2-705761
- Veröffentlichungsdatum Qucosa
- 20.05.2020
- Dokumenttyp
- Artikel
- Sprache des Dokumentes
- Englisch
- Lizenz / Rechtehinweis