- AutorIn
- Michael G. Haase Technische Universität Dresden, Department of Pediatric Surgery, University Hospital Carl Gustav Carus
- Peter GeyerTechnische Universität Dresden, Department of Radiotherapy and Radiation Oncology, University Hospital Carl Gustav Carus
- Guido FitzeTechnische Universität Dresden, Department of Pediatric Surgery, University Hospital Carl Gustav Carus
- Gustavo B. Baretton
- Titel
- Down-regulation of Heat Shock Protein HSP90ab1 in Radiation-damaged Lung Cells other than Mast Cells
- Zitierfähige Url:
- https://nbn-resolving.org/urn:nbn:de:bsz:14-qucosa2-355515
- Quellenangabe
- Journal of Histochemistry & Cytochemistry Erscheinungsort: London
Verlag: Sage
Erscheinungsjahr: 2014
Jahrgang: 62
Heft: 5
Seiten: 355-368
E-ISSN: 1551-5044 - Erstveröffentlichung
- 2014
- Abstract (EN)
- Ionizing radiation (IR) leads to fibrosing alveolitis (FA) after a lag period of several weeks to months. In a rat model, FA starts at 8 weeks after IR. Before that, at 5.5 weeks after IR, the transcription factors Sp1 (stimulating protein 1) and AP-1 (activator protein 1) are inactivated. To find genes/proteins that were down-regulated at that time, differentially expressed genes were identified in a subtractive cDNA library and verified by quantitative RT-PCR (reverse transcriptase polymerase chain reaction), western blotting and immunohistochemistry (IH). The mRNA of the molecular chaperone HSP90AB1 (heat shock protein 90 kDa alpha, class B member 1) was down-regulated 5.5 weeks after IR. Later, when FA manifested, HSP90ab1 protein was down-regulated by more than 90% in lung cells with the exception of mast cells. In most mast cells of the normal lung, both HSP90ab1 and HSP70, another major HSP, show a very low level of expression. HSP70 was massively up-regulated in all mast cells three months after irradiation whereas HSP90AB1 was up-regulated only in a portion of mast cells. The strong changes in the expression of central molecular chaperones may contribute to the well-known disturbance of cellular functions in radiation-damaged lung tissue. (J Histochem Cytochem 62:355–368, 2014)
- Andere Ausgabe
- Link zum Artikel, der zuerst in der Zeitschrift 'Journal of Histochemistry & Cytochemistry' erschienen ist.
DOI: 10.1369/0022155414529133 - Freie Schlagwörter (DE)
- fibrosierende Alveolitis, Hitzeschockprotein, HSP90ab1, HSP70, Mastzellen, Strahlenschäden
- Freie Schlagwörter (EN)
- fibrosing alveolitis, heat shock protein, HSP90ab1, HSP70, mast cells, radiation damage
- Klassifikation (DDC)
- 540
- 610
- Verlag
- Sage, London
- Förder- / Projektangaben
- Deutsche Forschungsgemeinschaft
ID: MU 1299/1-1
Bundesministerium für Bildung und Forschung
ID: 01ZZ9604 - Version / Begutachtungsstatus
- publizierte Version / Verlagsversion
- URN Qucosa
- urn:nbn:de:bsz:14-qucosa2-355515
- Veröffentlichungsdatum Qucosa
- 30.09.2019
- Dokumenttyp
- Artikel
- Sprache des Dokumentes
- Englisch
- Lizenz / Rechtehinweis