- AutorIn
- Maria Gaertner Technische Universität Dresden, Faculty of Medicine Carl Gustav Carus, Department of Anesthesiology and Intensive Care Medicine, Clinical Sensoring and Monitoring
- Kerstin Schirrmann
- Christian SchnabelTechnische Universität Dresden, Faculty of Medicine Carl Gustav Carus, Department of Anesthesiology and Intensive Care Medicine, Clinical Sensoring and Monitoring
- Sven Meissner
- Ulrich Kertzscher
- Lars Kirsten
- Edmund Koch
- Titel
- Toward a comprehensive interpretation of intravital microscopy images in studies of lung tissue dynamics
- Zitierfähige Url:
- https://nbn-resolving.org/urn:nbn:de:bsz:14-qucosa2-353059
- Quellenangabe
- Journal of Biomedical Optics Herausgeber: Brian W. Pogue
Erscheinungsort: Bellingham, Wash.
Verlag: SPIE
Erscheinungsjahr: 2015
Jahrgang: 20
Heft: 6
Seiten: 066009-1-066009-10
ISSN: 1560-2281 - Erstveröffentlichung
- 2015
- Abstract (EN)
- Intravital microscopy (IVM) is a well-established imaging technique for real-time monitoring of microscale lung tissue dynamics. Although accepted as a gold standard in respiratory research, its characteristic image features are scarcely understood, especially when trying to determine the actual position of alveolar walls. To allow correct interpretation of these images with respect to the true geometry of the lung parenchyma, we analyzed IVM data of alveoli in a mouse model in comparison with simultaneously acquired optical coherence tomography images. Several IVM characteristics, such as double ring structures or disappearing alveoli in regions of liquid filling, could be identified and related to the position of alveoli relative to each other. Utilizing a ray tracing approach based on an idealized geometry of the mouse lung parenchyma, two major reflection processes could be attributed to the IVM image formation: partial reflection and total internal reflection between adjacent alveoli. Considering the origin of the reflexes, a model was developed to determine the true position of alveolar walls within IVM images. These results allow thorough understanding of IVM data and may serve as a basis for the correction of alveolar sizes for more accurate quantitative analysis within future studies of lung tissue dynamics.
- Andere Ausgabe
- Link zum Artikel, der zuerst in der Zeitschrift Journal of Biomedical Optics' erschienen ist.
DOI: 10.1117/1.JBO.20.6.066009 - Freie Schlagwörter (DE)
- Bildanalyse, optische Kohärenztomographie, intravitale Mikroskopie, in-vivo-Mikroskopie, alveolare Größe, Bildgebung von Lungengewebe
- Freie Schlagwörter (EN)
- image analysis, optical coherence tomography, intravital microscopy, in vivo microscopy, alveolar size, lung tissue imaging
- Klassifikation (DDC)
- 530, 610
- Verlag
- SPIE, Bellingham, Wash.
- Förder- / Projektangaben
- Deutsche Forschungsgemeinschaft
Protective Artificial RespirationID: AF 3/33-2
Deutsche ForschungsgemeinschaftProtective Artificial Respiration
ID: KO 1814/6-1
Deutsche Forschungsgemeinschaft Protective Artificial RespirationID: KO 1814/6-2 - Version / Begutachtungsstatus
- publizierte Version / Verlagsversion
- URN Qucosa
- urn:nbn:de:bsz:14-qucosa2-353059
- Veröffentlichungsdatum Qucosa
- 09.09.2019
- Dokumenttyp
- Artikel
- Sprache des Dokumentes
- Englisch
- Lizenz / Rechtehinweis