- AutorIn
- Jürgen Czarske Laboratory for measurement and sensor system technique, Technische Universität Dresden
- Katrin PhilippLaboratory for measurement and sensor system technique, Technische Universität Dresden
- Nektarios KoukourakisLaboratory for measurement and sensor system technique, Technische Universität Dresden
- Titel
- Structured illumination 3D microscopy using adaptive lenses and multimode fibers
- Zitierfähige Url:
- https://nbn-resolving.org/urn:nbn:de:bsz:14-qucosa2-349804
- Konferenz
- SPIE Digital Optical Technologies. Munich, 25-29 June 2017
- Quellenangabe
- Digital Optical Technologies 2017
Herausgeber: Bernard C. Kress, Wolfgang Osten, H. Paul Urbach
Erscheinungsort: Bellingham, Wash.
Verlag: SPIE
Erscheinungsjahr: 2017
Titel Schriftenreihe: Proceedings of SPIE
Bandnummer Schriftenreihe: 10335 - Erstveröffentlichung
- 2017
- Abstract (EN)
- Microscopic techniques with high spatial and temporal resolution are required for in vivo studying biological cells and tissues. Adaptive lenses exhibit strong potential for fast motion-free axial scanning. However, they also lead to a degradation of the achievable resolution because of aberrations. This hurdle can be overcome by digital optical technologies. We present a novel High-and-Low-frequency (HiLo) 3D-microscope using structured illumination and an adaptive lens. Uniform illumination is used to obtain optical sectioning for the high-frequency (Hi) components of the image, and nonuniform illumination is needed to obtain optical sectioning for the low-frequency (Lo) components of the image. Nonuniform illumination is provided by a multimode fiber. It ensures robustness against optical aberrations of the adaptive lens. The depth-of-field of our microscope can be adjusted a-posteriori by computational optics. It enables to create flexible scans, which compensate for irregular axial measurement positions. The adaptive HiLo 3D-microscope provides an axial scanning range of 1 mm with an axial resolution of about 4 microns and sub-micron lateral resolution over the full scanning range. In result, volumetric measurements with high temporal and spatial resolution are provided. Demonstration measurements of zebrafish embryos with reporter gene-driven fluorescence in the thyroid gland are presented.
- Andere Ausgabe
- Link zum Artikel, der zuerst in der Zeitschrift 'Proceedings of SPIE' erschienen ist.
DOI: 10.1117/12.2270301 - Freie Schlagwörter (DE)
- Dreidimensionale Mikroskopie, adaptive Optik, Speckle-Musterauswertung
- Freie Schlagwörter (EN)
- three dimensional microscopy, adaptive optics, speckle pattern evaluation
- Klassifikation (DDC)
- 620
- Verlag
- SPIE, Bellingham, Wash.
- Förder- / Projektangaben
- Deutsche Forschungsgemeinschaft ID: Cz55-32
- Version / Begutachtungsstatus
- publizierte Version / Verlagsversion
- URN Qucosa
- urn:nbn:de:bsz:14-qucosa2-349804
- Veröffentlichungsdatum Qucosa
- 13.08.2019
- Dokumenttyp
- Konferenzbeitrag
- Sprache des Dokumentes
- Englisch
- Lizenz / Rechtehinweis