- AutorIn
- Shan Cao Qilu University of Technology, School of Light Industry and Engineering, Shandong, China#Tianjin University of Science and Technology, College of Biotechnology, Key Laboratory of Industrial Fermentation Microbiology, Tianjin, China
- J. SongQilu University of Technology, School of Light Industry and Engineering, Shandong, China
- Y. ShenQilu University of Technology, School of Light Industry and Engineering, Shandong, China
- Q. Xin
- Y. C. Li
- Y. Li
- Titel
- Development and Investigation of Low Collagen Degradability Unhairing Enzyme by Gene Modification
- Zitierfähige Url:
- https://nbn-resolving.org/urn:nbn:de:bsz:14-qucosa2-344081
- Konferenz
- XXXV IULTCS Congress Dresden 2019 / 8th Freiberg Leather Days. Dresden, 25. - 28. Juni 2019
- Quellenangabe
- XXXV IULTCS Congress 2019 - Proceedings
Herausgeber: Dr. Dietrich Tegtmeyer, Dr. Michael Meyer
Erscheinungsort: Dresden
Verlag: Verein für Gerberei-Chemie und -Technik e. V., Freiberg, Forschungsinstitut für Leder und Kunststoffbahnen (FILK) gGmbH, Freiberg
Erscheinungsjahr: 2019 - Erstveröffentlichung
- 2019
- Abstract (EN)
- Content: Unhairing process brought serious pollution, and enzyme application for replacing polluting chemicals in unhairing process attracted much attention in recent years. However, the unhairing enzymes haven’t been accepted widely in actual production due to low purity, complex composition and poor stability. To solve these problems, unhairing enzyme is suggested to be improved by genetic modification in this research. The High-keratinase-producing gene (KerT), which was extracted from B. amyloliquefaciens TCCC11319, was introduced into the B.subtilis WB600 by heterologous expression. Because Bacillus subtilis WB600 is deficient in six extracellular proteases, this process successfully reduced the collagenolytic protease content in crude broth as well as improved the keratinase content. Meantime, the recombinant KerT produced by B.subtilis WB600 had the obviously unhairing effect to remove hairs. The results showed that the collagen degradability of recombinant KerT was slightly and it did not cause any adverse effects on the hide quality. This research will contribute to the development of unhairing enzyme, and the novel unhairing enzyme might be applied as the key factor for the advanced cleaning biotechnology in leather production process. Take-Away: 1. The keratinase gene KerT was firstly reported and analyzed which was extracted from B. amyloliquefaciens TCCC11319. 2. The collagenolytic protease activity of unhairing enzyme was successfully inhibited by heterologous expression of kerT. 3. The unhairing effect of this novel unhairing enzyme was similar to current Sulphur-lime method without damaging hide structure.
- Freie Schlagwörter (EN)
- KerT gene, Enzyme Unhairing, Low collagen degradability, B.subtilis WB600, Heterologous expression
- Klassifikation (DDC)
- 620
- Klassifikation (RVK)
- ZS 7250
- Verlag
- Verein für Gerberei-Chemie und -Technik e. V., Freiberg
- Forschungsinstitut für Leder und Kunststoffbahnen (FILK) gGmbH, Freiberg
- Sonstige beteiligte Institution
- International Union of Leather Technologists and Chemists Societies
- Version / Begutachtungsstatus
- publizierte Version / Verlagsversion
- URN Qucosa
- urn:nbn:de:bsz:14-qucosa2-344081
- Veröffentlichungsdatum Qucosa
- 05.07.2019
- Dokumenttyp
- Konferenzbeitrag
- Sprache des Dokumentes
- Englisch
- Lizenz / Rechtehinweis