- AutorIn
- Sebastian Thieme
- Alexander Holzbaur
- Ralf Wiedemuth
- Aline Binner
- Katrin Navratiel
- Pof. Konstantinos Anastassiadis
- Dr. med. Sebastian Brenner
- Cornelia Richter
- Titel
- The Dox-pDC - A murine conditionally immortalized plasmacytoid dendritic cell line with native immune profile
- Zitierfähige Url:
- https://nbn-resolving.org/urn:nbn:de:bsz:14-qucosa-234947
- Quellenangabe
- PLOS ONE (2018), 13(2). S. 1-10. ISSN: 1932-6203. DOI: 10.1371/journal.pone.0192437.
- Erstveröffentlichung
- 2018
- Abstract (EN)
- Plasmacytoid dendritic cells (pDC) constitute a very rare blood cell population and play a significant role in immune response and immune-mediated disorders. Investigations on primary pDCs are hindered not only due to their rarity but also because they represent a heterogeneous cell population which is difficult to culture ex vivo. We generated a conditionally immortalized pDC line (Dox-pDC) from mice with Doxycycline-inducible SV40 Large T Antigen with a comparable immune profile to primary pDCs. The Dox-pDC secrete pro- and anti-inflammatory cytokines upon Toll-like receptor 9 stimulation and upregulate their MHCI, MHCII and costimulatory molecules. Further, the Dox-pDC activate and polarize naïve T cells in vivo and in vitro in response to the model antigen Ovalbumin. Due to their long-term culture stability and their robust proliferation Dox-pDC represent a reliable alternative to primary mouse pDC.
- Andere Ausgabe
- DOI: 10.1371/journal.pone.0192437
- Link zum Artikel, der zuerst in der Zeitschrift 'PLOS ONE' erschienen ist.
Link: https://doi.org/10.1371/journal.pone.0192437 - Freie Schlagwörter (DE)
- Plasmazytoide dendritische Zellen, Blutzellpopulation, TU Dresden, Publikationsfonds
- Freie Schlagwörter (EN)
- Plasmacytoid dendritic cells, blood cell population, TU Dresden, Publishing Fund
- Klassifikation (DDC)
- 610
- Klassifikation (RVK)
- XA 10000
- Verlag
- Public Library of Science, San Francisco, California, US
- Förder- / Projektangaben
- URN Qucosa
- urn:nbn:de:bsz:14-qucosa-234947
- Veröffentlichungsdatum Qucosa
- 11.06.2018
- Dokumenttyp
- Artikel
- Sprache des Dokumentes
- Englisch
- Lizenz / Rechtehinweis
- CC BY 4.0