- AutorIn
- Prof. Dr. Claudia Waskow
- Malte von Bonin
- Martin Wermke
- Kadriye Nehir Cosgun
- Christian Thiede
- Martin Bornhauser
- Gerard Wagemaker
- Titel
- In Vivo Expansion of Co-Transplanted T Cells Impacts on Tumor Re-Initiating Activity of Human Acute Myeloid Leukemia in NSG Mice
- Zitierfähige Url:
- https://nbn-resolving.org/urn:nbn:de:bsz:14-qucosa-191633
- Quellenangabe
- PLoS ONE, Volume 8(2013) Number 4: e60680, ISSN 1932-6203
- Erstveröffentlichung
- 2013
- Abstract (EN)
- Human cells from acute myeloid leukemia (AML) patients are frequently transplanted into immune-compromised mouse strains to provide an in vivo environment for studies on the biology of the disease. Since frequencies of leukemia re-initiating cells are low and a unique cell surface phenotype that includes all tumor re-initiating activity remains unknown, the underlying mechanisms leading to limitations in the xenotransplantation assay need to be understood and overcome to obtain robust engraftment of AML-containing samples. We report here that in the NSG xenotransplantation assay, the large majority of mononucleated cells from patients with AML fail to establish a reproducible myeloid engraftment despite high donor chimerism. Instead, donor-derived cells mainly consist of polyclonal disease-unrelated expanded co-transplanted human T lymphocytes that induce xenogeneic graft versus host disease and mask the engraftment of human AML in mice. Engraftment of mainly myeloid cell types can be enforced by the prevention of T cell expansion through the depletion of lymphocytes from the graft prior transplantation.
- Andere Ausgabe
- DOI: 10.1371/journal.pone.0060680
- Link zur Originalpublikation in der Zeitschrift PLoS ONE.
Link: http://dx.doi.org/10.1371/journal.pone.0060680 - Freie Schlagwörter (DE)
- Blut, Knochenmarktransplantation
- Freie Schlagwörter (EN)
- T cells, Bone marrow transplantation, Bone marrow cells, Acute myeloid leukemia, White blood cells, Blood, T cell receptors, Spleen
- Klassifikation (DDC)
- 610
- Klassifikation (RVK)
- XA 10000
- Verlag
- Public Library of Science, Lawrence, Kan.
- URN Qucosa
- urn:nbn:de:bsz:14-qucosa-191633
- Veröffentlichungsdatum Qucosa
- 18.01.2016
- Dokumenttyp
- Artikel
- Sprache des Dokumentes
- Englisch
- Lizenz / Rechtehinweis
- CC BY 4.0