Abstract
In July 2016, an aggressive syndrome of tomato yellow leaf curl disease was reported in Sicily in tomato plants carrying the Ty-1 resistance gene. A total of 34 samples were collected and analyzed. Twenty-seven out of the 34 samples analyzed appeared to contain only recombinant molecules. One full sequence was obtained after cloning. Alignments and plot similarity analysis showed that the genome of the recombinant, named TYLCV-IL[IT:Sic23:16], was mostly derived from tomato yellow leaf curl virus (TYLCV), with a small region of 132 nucleotides in the non-coding region between the stem-loop and the start of the V2 ORF replaced by 124 nucleotides derived from a virus of a different species, tomato yellow leaf curl Sardinia virus. All plants in which the new recombinant was detected belonged to resistant tomato cultivars.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Belabess Z, Dallot S, El-Montaser S, Granier M, Majde M, Tahiri A, Blenzar A, Urbino C, Peterschmitt M (2015) Monitoring the dynamics of emergence of a non-canonical recombinant of Tomato yellow leaf curl virus and displacement of its parental viruses in tomato. Virology 486:291–306
Belabess Z, Urbino C, Granier M, Tahiri A, Blenzar A, Peterschmitt M (2018) The typical RB76 recombination breakpoint of the invasive recombinant tomato yellow leaf curl virus of Morocco can be generated experimentally but is not positively selected in tomato. Virus Res 243:44–51
Davino S, Napoli C, Davino M, Accotto GP (2006) Spread of Tomato yellow leaf curl virus in Sicily: partial displacement of another geminivirus originally present. Eur. J. Plant Pathol 114:293–299
Davino S, Miozzi L, Panno S, Rubio L, Davino M, Accotto GP (2012) Recombination profiles between Tomato yellow leaf curl virus and Tomato yellow leaf curl Sardinia virus in laboratory and field conditions: evolutionary and taxonomic implications. J Gen Virol 93:2712–2717
Garcia-Andres S, Accotto GP, Navas-Castillo J, Moriones E (2007) Founder effect, plant host, and recombination shape the emergent population of begomoviruses that cause the tomato yellow leaf curl disease in the Mediterranean basin. Virology 359:302–312
Noris E, Hidalgo E, Accotto GP, Moriones E (1994) High similarity among the tomato yellow leaf curl virus isolates from the west Mediterranean basin: the nucleotide sequence of an infectious clone from Spain. Arch Virol 135:165–170
Rubio L, Ayllon M, Guerri J, Pappu H, Niblet C, Moreno P (1996) Differentiation of Citrus tristeza closterovirus (CTV) isolates by single-strand conformation polymorphism analysis of the coat protein gene. Ann Appl Biol 129:479–489
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory Press, New York
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
None to declare.
Funding sources
None to declare.
Additional information
Handling Editor: Jesus Navas-Castillo.
Rights and permissions
About this article
Cite this article
Panno, S., Caruso, A.G. & Davino, S. The nucleotide sequence of a recombinant tomato yellow leaf curl virus strain frequently detected in Sicily isolated from tomato plants carrying the Ty-1 resistance gene. Arch Virol 163, 795–797 (2018). https://doi.org/10.1007/s00705-017-3674-9
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00705-017-3674-9