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Non-clonal Dissemination of Extended-Spectrum Beta-Lactamase-Producing Pseudomonas aeruginosa Strains of Clinical Origin

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Abstract

Monitoring the emergence and transmission of Pseudomonas aeruginosa strains is important for infection control worldwide. The aim of this study is to determine the genetic diversity of 47 extended-spectrum beta-lactamase-producing P. aeruginosa strains of clinical origin by enterobacterial repetitive intergenic consensus PCR (ERIC-PCR) and also to ascertain the presence of class 1 integrons among them. ERIC sequences are 127 bp imperfect palindromes that occur in multiple copies in the genomes of enteric bacteria. The ERIC-PCR profile generated showed polymorphism in the studied strains. Based on the ERIC-PCR profile, P. aeruginosa strains were grouped into 23 types. The major genotype, designated as type L1, accounted for 10.6% (5/47) of samples. Class 1 integrons were detected in 8 of the 47 clinical isolates. Further, the integron-carrying isolates were found to display higher minimum inhibitory concentration against tested antibiotics. Optimization of antimicrobial use and control of infection is recommended to prevent the increase in the population of drug-resistant organisms.

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Acknowledgements

Shaikh S was supported by INSPIRE grant from the Department of Science and Technology, New Delhi, India (Grant Number: IF130056), which is sincerely acknowledged. Shazi Shakil thanks all of the staff of KACST Technology Innovation Center for Personalized Medicine at King Abdulaziz University, Jeddah, Saudi Arabia, for continued support.

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Authors and Affiliations

  1. Department of Biosciences, Integral University, Lucknow, 226026, India

    Sibhghatulla Shaikh & Neelam Pathak

  2. Department of Pharmacology and toxicology, College of Pharmacy, University of Hail, Hail, Saudi Arabia

    Syed Mohd. Danish Rizvi

  3. KACST Technology Innovation Center for Personalized Medicine, King Abdulaziz University, Jeddah, Saudi Arabia

    Shazi Shakil

  4. Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, King Abdulaziz University, P.O. Box 80216, Jeddah, 21589, Saudi Arabia

    Shazi Shakil

  5. Center of Excellence in Genomic Medicine Research, King Abdulaziz University, P.O. Box 80216, Jeddah, Saudi Arabia

    Shazi Shakil

  6. Department of Bio-engineering, Integral University, Lucknow, 226026, India

    Adnan Ahmad

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  1. Sibhghatulla Shaikh
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  2. Syed Mohd. Danish Rizvi
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  3. Shazi Shakil
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  4. Adnan Ahmad
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  5. Neelam Pathak
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Correspondence to Shazi Shakil.

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Shaikh, S., Rizvi, S.M.D., Shakil, S. et al. Non-clonal Dissemination of Extended-Spectrum Beta-Lactamase-Producing Pseudomonas aeruginosa Strains of Clinical Origin. Iran J Sci Technol Trans Sci 41, 1011–1015 (2017). https://doi.org/10.1007/s40995-017-0340-8

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  • DOI: https://doi.org/10.1007/s40995-017-0340-8

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