Abstract
This report describes a routine method taking less than 20 min to quantify cyanogenic glycosides such as linustatin and neolinustatin from flaxseeds (Linum usitatissimum L.) using 1H nuclear magnetic resonance. After manual dehulling, a higher linustatin content was shown in the almond fraction, while neolinustatin and total cyanogenic glycoside contents were significantly higher in hulls. Linustatin and neolinustatin were quantified in seven cultivars grown in two locations in three different years. Linustatin, neolinustatin, and total cyanogenic glycosides ranged between 91 and 267 mg/100 g, 78–272 mg/100 g, and 198–513 mg/100 g dry weight flaxseeds, respectively. NMR revealed differences of up to 70% between samples with standard deviation variations lower than 6%. This study shows that NMR is a very suitable tool to perform flaxseed varietal selection for the cyanogenic glycoside content.
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Acknowledgments
The authors wish to thank the GRANOLIN consortium. R. Roulard wishes to thank OSEO for the award of a PhD grant. The authors are grateful for financial support from the European Regional Development Fund (ERDF). F. Mesnard wishes to acknowledge COST action FA 1006. The authors wish to thank Beatriz Oves Suarez for her participation in statistical analyses.
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Roulard, R., Fontaine, JX., Jamali, A. et al. Use of qNMR for speciation of flaxseeds (Linum usitatissimum) and quantification of cyanogenic glycosides. Anal Bioanal Chem 409, 7011–7026 (2017). https://doi.org/10.1007/s00216-017-0637-7
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DOI: https://doi.org/10.1007/s00216-017-0637-7