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Comparison of Selected Protein Levels in Tumour and Surgical Margin in a Group of Patients with Oral Cavity Cancer

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Abstract

Oral cavity cancer belongs to head-and-neck squamous cell carcinoma group. The purpose of the study was to assess the levels of certain proteins in a tumour and surgical margin in a group of patients with oral cavity cancer. The levels of DAPK1, MGMT, CDH1, SFRP1, SFRP2, RORA, TIMP3, p16, APC and RASSF1 proteins were measured by ELISA in tissue homogenates. The protein levels of DAPK1, MGMT, CDH1, SFRP2 and RASSF1 were significantly higher in tumour tissue than in the margin, contrary to TIMP3 which was lower in the tumour itself. DAPK1 level in the tumour was significantly higher in females than in males, the MGMT and p16 levels were lower in the tumours with lymph node metastasis (N1 + N2) than in N0 samples. The CDH1 expression was higher in a group with smoking habits, whereas TIMP3 was lower in this group. Changes in the levels of proteins in tumour and surgical margin may be either reflective of tumour occurrence and development, or they might be also responsible for the progress and reoccurrence of the disease. Levels of the studied proteins might be good prognostic factors; however, further studies are required.

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Acknowledgements

The study was based on personal academic funds without any support from external organisations or companies.

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Authors and Affiliations

  1. Department of Medical and Molecular Biology, School of Medicine with the Division of Dentistry in Zabrze, Medical University of Silesia in Katowice, Jordana 19 Str., 41-808, Zabrze, Poland

    Joanna Katarzyna Strzelczyk, Karolina Gołąbek, Piotr Cuber, Martyna Fronczek & Zofia Ostrowska

  2. Clinic of Oncological and Reconstructive Surgery, Maria Sklodowska–Curie Memorial Cancer Center and Institute of Oncology, Gliwice Branch, Gliwice, Poland

    Łukasz Krakowczyk

  3. Department of Statistics, Department of Instrumental Analysis, School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia in Katowice, Sosnowiec, Poland

    Aleksander Jerzy Owczarek & Piotr Choręża

  4. Department of Biochemistry, School of Medicine with the Division of Dentistry in Zabrze, Medical University of Silesia in Katowice, Zabrze, Poland

    Edyta Hudziec

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  1. Joanna Katarzyna Strzelczyk
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  2. Karolina Gołąbek
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Strzelczyk, J.K., Gołąbek, K., Cuber, P. et al. Comparison of Selected Protein Levels in Tumour and Surgical Margin in a Group of Patients with Oral Cavity Cancer. Biochem Genet 55, 322–334 (2017). https://doi.org/10.1007/s10528-017-9799-4

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  • DOI: https://doi.org/10.1007/s10528-017-9799-4

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